| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 47,828 | A→G | 85.5% | V20V (GTA→GTG) | kefC → | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 47,828 | 0 | A | G | 85.5% | 141.1 / 14.9 | 55 | V20V (GTA→GTG) | kefC | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base G (31/16); total (33/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
AGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGG > W3110S.gb/47760‑47893 | aggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc < 1:2848987/71‑1 (MQ=255) ggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGcc > 1:241478/1‑70 (MQ=255) ggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAccc < 1:2653961/71‑1 (MQ=255) ggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAccc < 1:2128146/71‑1 (MQ=255) ggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAccc < 1:1894405/71‑1 (MQ=255) ggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc > 1:1284010/1‑70 (MQ=255) gCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAccc < 1:1835819/70‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGcc < 1:2682654/64‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGcc < 1:798980/64‑1 (MQ=255) tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGcc < 1:2066243/64‑1 (MQ=255) catGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg > 1:3329159/1‑71 (MQ=255) catGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg > 1:2390785/1‑71 (MQ=255) atGGGTAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGTAGCGCTGATTGTGCCCATTGCgg > 1:720102/1‑70 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGGCCATTGCgg > 1:3000510/1‑70 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg < 1:962950/70‑1 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg > 1:2981026/1‑70 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg > 1:471952/1‑70 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg > 1:2085280/1‑70 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg > 1:669505/1‑70 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg > 1:1593470/1‑70 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCg > 1:1441601/1‑69 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCg > 1:1270372/1‑69 (MQ=255) atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCg > 1:1316776/1‑69 (MQ=255) gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTa > 1:633071/1‑57 (MQ=255) gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTa > 1:3069379/1‑57 (MQ=255) gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTa > 1:2448861/1‑57 (MQ=255) gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACg > 1:1716611/1‑71 (MQ=255) aTACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg < 1:3460444/60‑1 (MQ=255) aTACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg < 1:2999935/60‑1 (MQ=255) aTACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGt < 1:354044/71‑1 (MQ=255) aTACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGt < 1:290377/71‑1 (MQ=255) tACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGcc < 1:194102/51‑1 (MQ=255) tACGCTGATTCAGGCGCTGATTTATATCGGTTCGGCAGCGCTGATTGTGcc < 1:3719893/51‑1 (MQ=38) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:3613651/1‑51 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:1913158/1‑51 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCa > 1:2918747/1‑51 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg > 1:923666/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg > 1:166287/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg > 1:1607227/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg > 1:2123630/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg > 1:2019876/1‑71 (MQ=255) cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg > 1:1967706/1‑71 (MQ=255) cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg < 1:2683061/71‑1 (MQ=255) cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg < 1:2612758/71‑1 (MQ=255) cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg < 1:2245335/71‑1 (MQ=255) tCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg > 1:1941088/1‑71 (MQ=255) cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCgg > 1:472748/1‑70 (MQ=255) cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCgg < 1:790274/70‑1 (MQ=255) aGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGt < 1:3016848/70‑1 (MQ=255) cTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTgg > 1:3167488/1‑71 (MQ=255) cTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTgg > 1:3627080/1‑71 (MQ=255) tGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg < 1:1860492/58‑1 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt > 1:3245642/1‑71 (MQ=255) tATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt > 1:2881862/1‑71 (MQ=255) gTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc < 1:99513/71‑1 (MQ=255) aGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCa > 1:1359080/1‑71 (MQ=255) aGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCa > 1:333733/1‑71 (MQ=255) aTTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTgg > 1:829829/1‑71 (MQ=255) | AGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGG > W3110S.gb/47760‑47893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |