| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 579,680 | G→A | 100% | intergenic (+371/‑377) | ybcW → / → nohB | hypothetical protein/DNA packaging protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 579,680 | 0 | G | A | 100.0% | 118.9 / NA | 43 | intergenic (+371/‑377) | ybcW/nohB | hypothetical protein/DNA packaging protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/22); total (21/22) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGTATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGTTTTTACGTTA > W3110S.gb/579616‑579726 | ggAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTc < 1:196086/70‑1 (MQ=25)ggAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTc < 1:1615882/70‑1 (MQ=25)ggAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTc < 1:3104729/70‑1 (MQ=25)ggAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTGCTGTTCTTAGTTGACATGAGGTTGCCCCATATTc < 1:321343/70‑1 (MQ=255) aaaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAgt < 1:2409385/71‑1 (MQ=21) aaaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAgt < 1:2823533/71‑1 (MQ=21) aaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAgtg > 1:1238666/1‑71 (MQ=21) aaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAgtg > 1:2323956/1‑71 (MQ=21) aaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAgtg > 1:793046/1‑71 (MQ=21) aaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAgtg > 1:40817/1‑71 (MQ=21) aaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAgtg > 1:483298/1‑71 (MQ=21) aaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTT‑ACATGAGGTTGCCCCATATTCAgtgt > 1:1644071/1‑71 (MQ=11) cGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCg > 1:3249271/1‑71 (MQ=21) cGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCg > 1:573946/1‑71 (MQ=21) tGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGa < 1:2770557/71‑1 (MQ=21) gCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATa > 1:593017/1‑53 (MQ=255) gCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTg > 1:1816347/1‑71 (MQ=21) gCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTg > 1:2626000/1‑71 (MQ=21) cGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGGTGCCCCATATTCAGTGTCGCTGATTTg < 1:2653780/70‑1 (MQ=255) tgttgttCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCt < 1:2448676/59‑1 (MQ=255) tgttgttCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCt < 1:2155083/59‑1 (MQ=255) tgttgttCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCt < 1:1465150/59‑1 (MQ=255) gttgttCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTc > 1:1941187/1‑71 (MQ=255) gttgttCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTc > 1:1936800/1‑71 (MQ=255) ttCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTCTGa < 1:169011/70‑1 (MQ=25) gttctgttctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGtttt < 1:2668841/71‑1 (MQ=21) ctgttctTAGTTGACATGAGGTTGCCCCATATTCAg > 1:59253/1‑36 (MQ=255) ctgttctTAGTTGACATGAGGTTGCCCCATATTCAg > 1:2765984/1‑36 (MQ=255) gttctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGTTTTTACGt > 1:2871498/1‑71 (MQ=21) ttctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCt < 1:1840218/40‑1 (MQ=255) ttctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCt < 1:496512/40‑1 (MQ=255) ttctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCt < 1:1473621/40‑1 (MQ=255) ttctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCt < 1:1496453/40‑1 (MQ=255) ttctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCt < 1:3068206/40‑1 (MQ=255) ttctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCt < 1:2413568/40‑1 (MQ=255) ttctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCCCt < 1:541460/40‑1 (MQ=14) ctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGa > 1:2076773/1‑40 (MQ=255) ctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGa > 1:498312/1‑40 (MQ=255) ctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGTTTTTACGtt > 1:915168/1‑69 (MQ=25) ctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGTTTTTACGtt > 1:1195448/1‑69 (MQ=25) ctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGTTTTTACGtt > 1:1368649/1‑69 (MQ=25) ctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGTTTTTACGTTa < 1:2458058/70‑1 (MQ=25) ctTAGTTGACATGAGGTTGCCCCATATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGTTTTTACGTTa < 1:2011996/70‑1 (MQ=25) | GGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGTATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGTTTTTACGTTA > W3110S.gb/579616‑579726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |