Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 614,426 C→T 76.6% L349L (CTC→CTT entF → enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb614,4260CT76.6% 119.6 / 38.0 64L349L (CTC→CTTentFenterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (3/12);  new base T (20/29);  total (23/41)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGC  >  W3110S.gb/614360‑614491
                                                                  |                                                                 
tGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAata                                                               <  1:1069306/71‑1 (MQ=255)
tGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAata                                                               <  1:874106/71‑1 (MQ=255)
tGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAata                                                               <  1:1197553/71‑1 (MQ=255)
tGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAata                                                               <  1:341234/71‑1 (MQ=255)
      aCAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAgg                                                         <  1:2088930/71‑1 (MQ=255)
      aCAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAgg                                                         <  1:3555239/71‑1 (MQ=255)
           ttGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTAttt                                                    <  1:3429983/71‑1 (MQ=255)
             gTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTg                                                   <  1:2724528/70‑1 (MQ=255)
             gTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTg                                                   <  1:410437/70‑1 (MQ=255)
              tCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGAt                                                 <  1:2948656/71‑1 (MQ=255)
              tCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGAt                                                 <  1:338809/71‑1 (MQ=255)
              tCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGAt                                                 <  1:1105157/71‑1 (MQ=255)
                         gggCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGa                                      <  1:1286945/71‑1 (MQ=255)
                         gggCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGa                                      <  1:1685680/71‑1 (MQ=255)
                                cGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCt                               <  1:1973442/71‑1 (MQ=255)
                                cGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCt                               <  1:3085538/71‑1 (MQ=255)
                                    aGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATg                           <  1:2010121/71‑1 (MQ=255)
                                    aGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATg                           <  1:661844/71‑1 (MQ=255)
                                    aGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATg                           <  1:3376010/71‑1 (MQ=255)
                                     ggTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTAttt                                                    <  1:1529587/45‑1 (MQ=255)
                                     ggTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTAttt                                                    <  1:2954315/45‑1 (MQ=255)
                                     ggTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTAttt                                                    <  1:1289540/45‑1 (MQ=255)
                                     ggTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTAttt                                                    <  1:1266569/45‑1 (MQ=255)
                                     ggTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTAttt                                                    <  1:666083/45‑1 (MQ=255)
                                     ggTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATg                           <  1:2432107/70‑1 (MQ=255)
                                     ggTGATGAACCGCTGTTTGGTCAGGTACTTAATATCAAGGTAttt                                                    <  1:2373488/45‑1 (MQ=38)
                                       tgatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTa                                               <  1:2387620/48‑1 (MQ=255)
                                       tgatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTa                                               <  1:2484075/48‑1 (MQ=255)
                                       tgatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTa                                               <  1:2379735/48‑1 (MQ=255)
                                       tgatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTa                                               <  1:1872939/48‑1 (MQ=255)
                                       tgatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTa                                               <  1:1873467/48‑1 (MQ=255)
                                        gatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGtt                         >  1:1388089/1‑69 (MQ=255)
                                         atgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTgg                                       <  1:2906405/54‑1 (MQ=255)
                                         atgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTgg                                       <  1:1555167/54‑1 (MQ=255)
                                         atgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTgg                                       <  1:1848205/54‑1 (MQ=255)
                                         atgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATg                           <  1:1738694/66‑1 (MQ=255)
                                         atgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAg                      >  1:619191/1‑71 (MQ=255)
                                         atgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAg                      >  1:879790/1‑71 (MQ=255)
                                         atgaACCGCTGATTGTTCCGGTACTTAATATCAAGGTAttt                                                    <  1:301558/41‑1 (MQ=37)
                                           gaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGa                                                  >  1:387416/1‑41 (MQ=255)
                                           gaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGa                                                  >  1:3539030/1‑41 (MQ=255)
                                           gaACCGATGTTTGGTCCGGTACTTAATATCAAGGTATTTGa                                                  >  1:2883/1‑41 (MQ=38)
                                            aaCCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGgcg                   >  1:2196669/1‑71 (MQ=255)
                                                   gTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGa                                      >  1:1184894/1‑45 (MQ=255)
                                                   gTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGa                                      >  1:122583/1‑45 (MQ=255)
                                                   gTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGa                                      >  1:2990423/1‑45 (MQ=255)
                                                   gTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGa                                      >  1:3573294/1‑45 (MQ=255)
                                                   gTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGa                                      >  1:1518411/1‑45 (MQ=255)
                                                    tttGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCaa                <  1:1562132/66‑1 (MQ=255)
                                                    tttGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCaa                <  1:848393/66‑1 (MQ=255)
                                                      tGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCAt          <  1:1788207/70‑1 (MQ=255)
                                                      tGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATa         >  1:1259655/1‑71 (MQ=255)
                                                      tGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATa         >  1:3161798/1‑71 (MQ=255)
                                                      tGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATa         >  1:388391/1‑71 (MQ=255)
                                                      tGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATa         >  1:917810/1‑71 (MQ=255)
                                                        gTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATAcc       <  1:1122906/71‑1 (MQ=255)
                                                          ccGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCt     >  1:2244524/1‑71 (MQ=255)
                                                          ccGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCt     >  1:2894470/1‑71 (MQ=255)
                                                          ccGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCt     >  1:2428568/1‑71 (MQ=255)
                                                             gTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATAcc       >  1:1850253/1‑66 (MQ=255)
                                                             gTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATAcc       >  1:1221006/1‑66 (MQ=255)
                                                             gTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATAcc       >  1:1020537/1‑66 (MQ=255)
                                                             gTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATAcc       >  1:2098635/1‑66 (MQ=255)
                                                             gTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGc  <  1:3502521/71‑1 (MQ=255)
                                                                  |                                                                 
TGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGC  >  W3110S.gb/614360‑614491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: