| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 669,029 | C→T | 51.4% | E37K (GAA→AAA) | ybeA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 669,029 | 0 | C | T | 51.4% | ‑5.5 / 50.6 | 35 | E37K (GAA→AAA) | ybeA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (6/11); new base T (14/4); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
TGCTCACCCTCTTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACC > W3110S.gb/668962‑669093 | tGCTCACCCTCTTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAAt > 1:1491555/1‑71 (MQ=255)tGCTCACCCTCTTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAAt > 1:1628488/1‑71 (MQ=255) ccTCTTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCg < 1:101457/71‑1 (MQ=255) ccTCTTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCg < 1:2103125/71‑1 (MQ=255) ctTTGTCGAGTATGCGCTTGATGTCCGTATTCTTGCCGCGTTTTCCGGCCGGAATTTTAAt > 1:2708556/1‑61 (MQ=255) ctTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTTAAt > 1:3537624/1‑61 (MQ=255) tttGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAgg > 1:189434/1‑71 (MQ=255) tGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAggg < 1:3609992/70‑1 (MQ=255) cgcTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAGGGCatat > 1:379922/1‑64 (MQ=255) cgcTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAGGGCatat > 1:804419/1‑64 (MQ=255) cgcTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAGGGCATATCTTTCg > 1:439215/1‑70 (MQ=255) cgcTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAGGGCATATCTTTCg > 1:814838/1‑70 (MQ=255) cTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGaa < 1:658108/71‑1 (MQ=255) tCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGa < 1:909884/46‑1 (MQ=255) ccGCATTCTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGa < 1:2250011/45‑1 (MQ=255) cGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGc < 1:245961/70‑1 (MQ=255) cGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGc < 1:30530/70‑1 (MQ=255) cGCATACTTGCCGCGTTTTCCGGCCGGAATTTTAATCa > 1:3065228/1‑38 (MQ=38) gCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAg < 1:239296/71‑1 (MQ=255) cTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAGGGCATATCTTTCGGAAAacga > 1:1085450/1‑61 (MQ=255) cTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAGGGCATATCTTTCGGAAAacga > 1:1410659/1‑61 (MQ=255) cTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAGGGCATATCTTTCGGAAAacga > 1:2979464/1‑61 (MQ=255) cTTGCCGCGTTTTCCGGCCGGAATTTTAATCAGCTCGAAGGGCATATCTTTCGGAAAacg > 1:1832339/1‑60 (MQ=255) cTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACt > 1:194432/1‑71 (MQ=255) cTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACt > 1:1411127/1‑71 (MQ=255) ttttCCGGCCGGAATTTTAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAAc > 1:1733954/1‑71 (MQ=255) ttCCGGCCGGAGTTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCg > 1:256864/1‑71 (MQ=255) ttCCGGCCGGAATTTTAATCAGCTCGAAGGGCATATCTTTCGGaaaa > 1:1583159/1‑47 (MQ=255) aaTTTTAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTAcc < 1:443093/70‑1 (MQ=255) aTTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTAcc > 1:1685692/1‑69 (MQ=255) tttCAATCAGCTCGAAGGGCATATCTTTCGGAAAacg < 1:2944144/37‑1 (MQ=255) tttCAATCAGCTCGAAGGGCATATCTTTCGGAAAacg < 1:3488815/37‑1 (MQ=255) tttCAATCAGCTCGAAGGGCATATCTTTCGGAAAacg < 1:2896430/37‑1 (MQ=255) tttCAATCAGCTCGAAGGGCATATCTTTCGGAAAacg < 1:1713901/37‑1 (MQ=255) tttCAATCAGCTCGAAGGGCATATCTTTCGGAAAacg < 1:20115/37‑1 (MQ=255) | TGCTCACCCTCTTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACC > W3110S.gb/668962‑669093 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |