| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 810,589 | T→G | 100% | M275R (ATG→AGG) | bioB → | biotin synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 810,589 | 0 | T | G | 83.4% | 32.1 / ‑1.4 | 12 | M275R (ATG→AGG) | bioB | biotin synthase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (3/7); total (4/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAA > W3110S.gb/810525‑810632 | atgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCtt < 1:2917096/71‑1 (MQ=255) ttACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCTAGGTGCtt < 1:2420862/60‑1 (MQ=255) aCGTGCGCCTTTCTGCCGGACGCTAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTa > 1:839764/1‑60 (MQ=255) tGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTAt < 1:2386061/58‑1 (MQ=255) cgcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTc > 1:997581/1‑71 (MQ=255) tCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAtttt > 1:1225788/1‑71 (MQ=255) gAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCaa < 1:1739059/71‑1 (MQ=255) aTGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCg < 1:1419552/48‑1 (MQ=255) aTGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCg < 1:1626582/48‑1 (MQ=255) aTGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCg < 1:3323653/48‑1 (MQ=255) tGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCaaa > 1:2848172/1‑65 (MQ=255) cgaacAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCg < 1:2906308/43‑1 (MQ=255) | ATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAA > W3110S.gb/810525‑810632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |