| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 827,597 | C→T | 100% | P26P (CCG→CCA) | ybhS ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 827,597 | 0 | C | T | 93.2% | 124.0 / 1.3 | 44 | P26P (CCG→CCA) | ybhS | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base T (28/13); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGA > W3110S.gb/827533‑827656 | tAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcg > 1:650100/1‑71 (MQ=255)tAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcg > 1:3049717/1‑71 (MQ=255)tAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcg > 1:2411772/1‑71 (MQ=255) aaTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgc > 1:3378139/1‑71 (MQ=255) aaTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgc > 1:3377778/1‑71 (MQ=255) aaTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgc > 1:3215055/1‑71 (MQ=255) aTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgcg < 1:2983993/71‑1 (MQ=255) aTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgcg < 1:132138/71‑1 (MQ=255) cGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACg < 1:3081058/71‑1 (MQ=255) cGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACg < 1:2505982/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGa < 1:830797/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGa < 1:2896638/71‑1 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGa < 1:761324/71‑1 (MQ=255) aCCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCt > 1:485654/1‑71 (MQ=255) aaTAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCcgcg > 1:1609677/1‑71 (MQ=255) agcagTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCttt < 1:3066363/71‑1 (MQ=255) gTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACg > 1:3286712/1‑71 (MQ=255) gggATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAgcgc > 1:314093/1‑71 (MQ=255) gATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCt > 1:1436154/1‑44 (MQ=255) gATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCAGAGCGccc > 1:936403/1‑71 (MQ=255) gATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGccc > 1:1175676/1‑71 (MQ=255) gATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGccc > 1:3189275/1‑71 (MQ=255) gATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGccc > 1:1622094/1‑71 (MQ=255) gATCACTACCGCAATCAGCCAGCTACTTAGATCGCGAACGATATGCCGCGTCTCTTTAACGCACAGCGccc > 1:2587221/1‑71 (MQ=255) aTCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGAt > 1:3340371/1‑41 (MQ=255) aTCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCg < 1:1078599/71‑1 (MQ=255) tCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCttt < 1:902129/55‑1 (MQ=255) tCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCttt < 1:3153121/55‑1 (MQ=255) tCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGcac < 1:1020007/62‑1 (MQ=255) tCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGcac < 1:3219251/62‑1 (MQ=255) tCACTACCGCAATCAGCCAGCTACTCGGATCGCCAACGATCTGCCGCGTCTCTTTAACGcac < 1:2556031/62‑1 (MQ=255) cTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCt < 1:1762169/39‑1 (MQ=255) cTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCt < 1:1620901/39‑1 (MQ=255) tACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc > 1:1162516/1‑71 (MQ=255) aCCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc > 1:3619933/1‑70 (MQ=255) aCCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc > 1:379477/1‑70 (MQ=255) aCCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc > 1:2763602/1‑70 (MQ=255) aCCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc > 1:2799518/1‑70 (MQ=255) aCCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc > 1:283083/1‑70 (MQ=255) cagccagcTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCcag > 1:3276171/1‑70 (MQ=255) cagccagcTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCcag > 1:114772/1‑70 (MQ=255) cagccagcTACTTGGATCGCGAACGATCTGCCGCGGCTCTTTAAc > 1:1925477/1‑45 (MQ=255) agccagcTACTTGGATCGCGAACGATCTGCCGCGt > 1:80760/1‑35 (MQ=255) agccagcTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCcagga > 1:758033/1‑71 (MQ=255) | TAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGA > W3110S.gb/827533‑827656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |