| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 65,135 | C→T | 100% | D216N (GAT→AAT) | polB ← | DNA polymerase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 65,135 | 0 | C | T | 89.3% | 71.6 / 1.9 | 28 | D216N (GAT→AAT) | polB | DNA polymerase II |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base T (11/14); total (11/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAA > W3110S.gb/65067‑65205 | cTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATTGt < 1:3561202/71‑1 (MQ=255)cTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATTGt < 1:1869335/71‑1 (MQ=255)cTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGt < 1:3305414/71‑1 (MQ=255)cTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGt < 1:187736/71‑1 (MQ=255) tttGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGTTGGCAAAc > 1:971116/1‑71 (MQ=255) agcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGTTGGCAAACCAgg < 1:1078216/70‑1 (MQ=255) ggCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGt > 1:867354/2‑52 (MQ=255) cGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAg > 1:2248312/1‑51 (MQ=255) cGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAg > 1:3239380/1‑51 (MQ=255) cGAACTGCACCACGTTCCAACCGATGATCACATCAGGATTGTa > 1:2716719/1‑43 (MQ=255) cGAACTGCACCACGTTCCAACCGATGATCACATCAGGATTGTa > 1:1191607/1‑43 (MQ=255) aaCTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGtt < 1:162612/44‑1 (MQ=255) aaCTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGtt < 1:1789447/44‑1 (MQ=255) aaCTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGtt < 1:1080067/44‑1 (MQ=255) aaCTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGtt < 1:3228882/44‑1 (MQ=255) aaCTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGtt < 1:3495826/44‑1 (MQ=255) aCTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGc < 1:797395/71‑1 (MQ=255) cTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGc > 1:1664850/1‑70 (MQ=255) cTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGc > 1:1455672/1‑70 (MQ=255) cTGCACCACGTTCCAACCGATGATCACATCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGc > 1:2343542/1‑70 (MQ=255) caccacGTTCCAACCGATGATCACATCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACt < 1:2939665/71‑1 (MQ=255) accacGTTCCAACCGATGATCACATCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACt < 1:1069823/70‑1 (MQ=255) tgatCACATCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACg < 1:1641291/71‑1 (MQ=255) tCACATCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTAt < 1:2785541/71‑1 (MQ=255) tCACATCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTAt < 1:956842/71‑1 (MQ=255) caTCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACt < 1:942677/48‑1 (MQ=255) caTCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGggcggc > 1:2196047/1‑57 (MQ=255) caTCAGGATTGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGggcggc > 1:162058/1‑57 (MQ=255) cGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGAGGGCGGCTGGCGACGTATTCCAGTTCGaaa > 1:360707/1‑71 (MQ=255) | CTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAA > W3110S.gb/65067‑65205 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |