| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 904,996 | G→A | 84.0% | intergenic (‑107/‑19) | ybjP ← / → ybjQ | predicted lipoprotein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 904,996 | 0 | G | A | 84.0% | 183.2 / 27.6 | 75 | intergenic (‑107/‑19) | ybjP/ybjQ | predicted lipoprotein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (3/9); new base A (38/25); total (41/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.04e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
CGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA > W3110S.gb/904929‑905062 | cGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:3408594/70‑1 (MQ=255)cGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:3046422/70‑1 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAAc > 1:21975/1‑70 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAAc > 1:2033585/1‑70 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAAc > 1:1601452/1‑70 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAAc > 1:3213278/1‑70 (MQ=255) taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAAc > 1:3078754/1‑70 (MQ=255) ataatGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAg > 1:3707613/1‑71 (MQ=255) atGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAg > 1:634204/1‑68 (MQ=255) atGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAg > 1:167323/1‑68 (MQ=255) atGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAg > 1:610006/1‑68 (MQ=255) atGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGgag < 1:253370/71‑1 (MQ=255) atGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGgag < 1:2276532/71‑1 (MQ=255) atGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGgag < 1:2303159/71‑1 (MQ=255) tGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGgaga < 1:1773891/71‑1 (MQ=255) tGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGgaga < 1:1158912/71‑1 (MQ=255) tGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGgaga < 1:2455628/71‑1 (MQ=255) tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAg > 1:1987669/1‑62 (MQ=255) tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat < 1:3164362/71‑1 (MQ=255) tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat < 1:2956896/71‑1 (MQ=255) tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat < 1:2664372/71‑1 (MQ=255) tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat < 1:2271367/71‑1 (MQ=255) tCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATg < 1:1185279/70‑1 (MQ=255) tCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATg < 1:2776816/70‑1 (MQ=255) cAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGc > 1:2665117/1‑70 (MQ=255) cAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGc > 1:993321/1‑70 (MQ=255) taataGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAAt > 1:1255299/1‑70 (MQ=255) ataGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:2305181/49‑1 (MQ=255) ataGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:624279/49‑1 (MQ=255) ataGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:1186868/49‑1 (MQ=255) ataGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAAttt < 1:2586889/70‑1 (MQ=255) taGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTc < 1:1415218/71‑1 (MQ=255) taGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGgaga < 1:2471213/54‑1 (MQ=255) aGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACaaa > 1:274281/1‑45 (MQ=255) gCGTATCAGTCTGATAATGCTTTTGAGATCGAAGTCTTAACAAACAAGGAGATCGATCATGCAATTTTCTa > 1:2971434/1‑71 (MQ=255) gCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTa > 1:2543020/1‑71 (MQ=255) gCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTa > 1:1469358/1‑71 (MQ=255) cGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTAc < 1:3535347/71‑1 (MQ=255) gTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACa < 1:50271/71‑1 (MQ=255) gTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACa < 1:1958481/71‑1 (MQ=255) gTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACa < 1:468283/71‑1 (MQ=255) tCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAAcc > 1:641209/1‑71 (MQ=255) tCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAAcc > 1:652430/1‑71 (MQ=255) tCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAAcc > 1:913556/1‑71 (MQ=255) tCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAAcc > 1:1453892/1‑71 (MQ=255) tCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAAcc > 1:1258996/1‑71 (MQ=255) tCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAAcc > 1:1823436/1‑71 (MQ=255) aGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAAccc > 1:3589508/1‑70 (MQ=255) tCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:858465/46‑1 (MQ=255) cTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat < 1:255061/47‑1 (MQ=255) aaTGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTc < 1:938315/54‑1 (MQ=255) aTGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTc > 1:3334902/1‑53 (MQ=255) aTGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTc > 1:247326/1‑53 (MQ=255) aTGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTc > 1:3364857/1‑53 (MQ=255) tGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAAc > 1:1032277/1‑58 (MQ=255) tGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAAc > 1:773608/1‑58 (MQ=255) gCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAg > 1:2597017/1‑71 (MQ=255) gCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAg > 1:411870/1‑71 (MQ=255) gCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAg > 1:3608706/1‑71 (MQ=255) gCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAg > 1:335397/1‑71 (MQ=255) gCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAg > 1:2290647/1‑71 (MQ=255) gCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAg > 1:2673445/1‑71 (MQ=255) gCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAg > 1:3113556/1‑71 (MQ=255) gCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAg > 1:169486/1‑71 (MQ=255) tGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCa < 1:1228832/70‑1 (MQ=255) tGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCa < 1:1502256/70‑1 (MQ=255) agaTCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAc < 1:590707/71‑1 (MQ=255) agaTCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAc < 1:1905667/71‑1 (MQ=255) aTCGAAGGCTTAACAAACAAGGAGATCGATCATTCAATTTTCTACAACCCCAActc < 1:3581233/56‑1 (MQ=255) aTCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAActc < 1:2046096/56‑1 (MQ=255) gAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACaa > 1:1104542/1‑44 (MQ=255) gAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACaa > 1:2999245/1‑44 (MQ=255) ggCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTg < 1:2523441/71‑1 (MQ=255) ggCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTg < 1:2519301/71‑1 (MQ=255) cTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:284528/71‑1 (MQ=255) cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:59205/71‑1 (MQ=255) ttAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa > 1:325744/1‑70 (MQ=255) ttAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa > 1:2416632/1‑70 (MQ=255) | CGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA > W3110S.gb/904929‑905062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |