| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 906,962 | G→A | 100% | H72Y (CAC→TAC) | ybjS ← | predicted NAD(P)H oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 906,962 | 0 | G | A | 97.3% | 109.5 / ‑3.8 | 37 | H72Y (CAC→TAC) | ybjS | predicted NAD(P)H oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (14/22); total (14/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGCGCGAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCA > W3110S.gb/906896‑907021 | tGGCGCGAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACca > 1:2279627/1‑70 (MQ=255) cgAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGt < 1:2605057/71‑1 (MQ=255) cgAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGt < 1:2483515/71‑1 (MQ=255) aCGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATc < 1:153298/71‑1 (MQ=255) aCGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATc < 1:963474/71‑1 (MQ=255) aCGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATc < 1:2885506/71‑1 (MQ=255) aCGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATc < 1:1737216/71‑1 (MQ=255) tCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGa > 1:1339106/1‑70 (MQ=255) tCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAg > 1:1499930/1‑71 (MQ=255) tCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAg > 1:2907853/1‑71 (MQ=255) tCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAg > 1:1209355/1‑71 (MQ=255) gAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGc < 1:2858914/70‑1 (MQ=255) gAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCa > 1:434331/1‑71 (MQ=255) aaaGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCAt < 1:1854854/71‑1 (MQ=255) cTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACtt < 1:373206/71‑1 (MQ=255) cTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACtt < 1:1504022/71‑1 (MQ=255) cTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACtt < 1:830878/71‑1 (MQ=255) tgttgtGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATc > 1:1742649/1‑50 (MQ=255) tgttgtGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATc > 1:1126899/1‑50 (MQ=255) tgttgtGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATc > 1:373908/1‑50 (MQ=255) tgttgtGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACtt < 1:835112/70‑1 (MQ=255) ttgtGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAg < 1:3577667/71‑1 (MQ=255) gtgtCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAAt > 1:2610052/1‑49 (MQ=255) gtgtCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAAt > 1:2189270/1‑49 (MQ=255) gTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc > 1:1353209/1‑71 (MQ=255) gTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc > 1:2655089/1‑71 (MQ=255) gTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc > 1:548089/1‑71 (MQ=255) tAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:769450/56‑1 (MQ=255) tAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1196969/56‑1 (MQ=255) tAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:2320126/56‑1 (MQ=255) tAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1680314/56‑1 (MQ=255) tAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCg < 1:3090411/71‑1 (MQ=255) tAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCg < 1:2927580/71‑1 (MQ=255) tAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCg < 1:770135/71‑1 (MQ=255) gCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCa < 1:3576828/71‑1 (MQ=255) gCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCa < 1:2232969/71‑1 (MQ=255) aGTACCACAGCGTATCAATGCCCGCGAGCATCACtt < 1:2604083/36‑1 (MQ=255) | TGGCGCGAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCA > W3110S.gb/906896‑907021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |