| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 913,779 | A→G | 79.0% | L153P (CTG→CCG) | hcp ← | hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 913,779 | 0 | A | G | 79.0% | 79.7 / 16.4 | 38 | L153P (CTG→CCG) | hcp | hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base G (16/14); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
TGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTG > W3110S.gb/913728‑913839 | tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGATAATGtt < 1:3707/71‑1 (MQ=255)tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGtt < 1:3521076/71‑1 (MQ=255)tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGtt < 1:2935803/71‑1 (MQ=255) cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCg > 1:3186108/1‑71 (MQ=255) cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCg > 1:2482115/1‑71 (MQ=255) cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCg > 1:2247648/1‑71 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGa > 1:857585/1‑39 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGa > 1:588984/1‑39 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGa > 1:1378630/1‑39 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGa > 1:1656896/1‑39 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATcgccg > 1:3467078/1‑70 (MQ=255) aCCTTTCAGGCCATACAGGCACATCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:2916516/71‑1 (MQ=255) tttCAGGCCATACAGGCACAGCGGAGGCAGGCCGAGAATGTTTTCGCCaa > 1:2937396/1‑50 (MQ=255) tttCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAAt > 1:1433600/1‑39 (MQ=255) tttCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCaa > 1:1102801/1‑50 (MQ=255) tttCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCaa > 1:741960/1‑50 (MQ=255) tttCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAg > 1:478507/1‑71 (MQ=255) tttCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAg > 1:3678201/1‑71 (MQ=255) tCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAg < 1:3363921/69‑1 (MQ=255) cAGGCCATATAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAg < 1:2059332/68‑1 (MQ=255) cATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaaa < 1:3322666/70‑1 (MQ=255) tACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGcc < 1:3718281/38‑1 (MQ=255) tACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGcc < 1:708857/38‑1 (MQ=255) tACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGcc < 1:1411229/38‑1 (MQ=255) tACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGcc < 1:3191143/38‑1 (MQ=255) tACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGcc < 1:1546258/38‑1 (MQ=255) tACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCa < 1:2042085/39‑1 (MQ=255) tACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCa < 1:3255296/39‑1 (MQ=255) tACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:173273/58‑1 (MQ=255) aCAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGcc < 1:456297/37‑1 (MQ=255) aCAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATc > 1:2656023/1‑41 (MQ=255) aCAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct > 1:77921/1‑71 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCaa > 1:2386527/1‑38 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCaa > 1:412761/1‑38 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCaa > 1:2125418/1‑38 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg < 1:2240848/71‑1 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg < 1:826729/71‑1 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg < 1:844738/71‑1 (MQ=255) cagcggACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTa < 1:2860377/58‑1 (MQ=255) cagcggACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTa < 1:2004204/58‑1 (MQ=255) | TGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTG > W3110S.gb/913728‑913839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |