| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 941,935 | C→T | 100% | T185T (ACC→ACT) | dmsA → | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 941,935 | 0 | C | T | 77.8% | 30.7 / 6.0 | 18 | T185T (ACC→ACT) | dmsA | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (7/7); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTATCTGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACCCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGACTACTCCTCCGC > W3110S.gb/941868‑942000 | tctatctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATAccc < 1:1500917/69‑1 (MQ=255)tctatctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATAccc < 1:2266433/69‑1 (MQ=255) tatctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACCCTGGt > 1:3469450/1‑71 (MQ=255) tctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTg < 1:983899/67‑1 (MQ=255) tctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTg < 1:692677/67‑1 (MQ=255) tctGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTg < 1:1793884/67‑1 (MQ=255) tGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGACCGCCGGGAAATACTCTGGTcgcg > 1:1224008/1‑71 (MQ=255) tGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGACCGCCGGGAAATACTCTGGTcgcg > 1:719997/1‑71 (MQ=255) gCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtgatg < 1:1894811/71‑1 (MQ=255) gCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACCCTGGTCGCGCGGCtgatg < 1:1904115/71‑1 (MQ=255) ggTACGCTGGGCGGCACCATGACCCGCACCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAActg > 1:2241892/1‑71 (MQ=255) ccATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtgatg < 1:1528946/50‑1 (MQ=255) ccATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtgatg < 1:3475636/50‑1 (MQ=255) ccATGACCCGCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCtgatg < 1:134486/50‑1 (MQ=255) gCTCCTGGCCGCCGGGAAATACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTAc > 1:1460453/1‑71 (MQ=255) gccgccGGGAAATACCCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGac < 1:2366083/70‑1 (MQ=255) gccgccGGGAAATACCCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGac < 1:1183206/70‑1 (MQ=255) aaaTACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTa > 1:3005961/1‑54 (MQ=255) aaTACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGACTACTCCTCcgc > 1:1042562/1‑71 (MQ=255) aaTACTCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGACTACTCCTCcgc > 1:3053090/1‑71 (MQ=255) | TCTATCTGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACCCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCATTACGGCGACTACTCCTCCGC > W3110S.gb/941868‑942000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |