| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,016,291 | T→C | 100% | V52A (GTA→GCA) | rmf → | ribosome modulation factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,016,291 | 0 | T | C | 97.0% | 106.4 / ‑3.9 | 33 | V52A (GTA→GCA) | rmf | ribosome modulation factor |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (8/24); total (8/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAC > W3110S.gb/1016227‑1016358 | tATCAGACGCTGAATCAAAGGTCACAAT‑‑‑‑‑‑‑‑GGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTg < 1:3445416/69‑1 (MQ=255)tATCAGACGCTGAATCAAAGGTCACAAT‑‑‑‑‑‑‑‑GGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTg < 1:2489916/69‑1 (MQ=255)tATCAGACGCTGAATCAAAGGTCACAAT‑‑‑‑‑‑‑‑GGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTg < 1:2003973/69‑1 (MQ=255)tATCAGACGCTGAATCAAAGGTCACAAT‑‑‑‑‑‑‑‑GGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTg < 1:1960987/69‑1 (MQ=255) aTCAGACGCTGAATCAAAGGTCACAAT‑‑‑‑‑‑‑‑GGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTg < 1:769437/68‑1 (MQ=255) tCAGACGCTGAATCAAAGGTCACAAT‑‑‑‑‑‑‑‑GGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTg < 1:2332383/67‑1 (MQ=255) aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGcc > 1:2465533/1‑71 (MQ=255) aTCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACATGGCAGTAATGGCCTGATTCTGt < 1:3716273/71‑1 (MQ=255) aTCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGt < 1:1082603/71‑1 (MQ=255) aTCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGt < 1:898163/71‑1 (MQ=255) aTCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGt < 1:1915268/71‑1 (MQ=255) aTCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGt < 1:1318895/71‑1 (MQ=255) cacaATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgcag > 1:809793/1‑45 (MQ=255) cacaATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTaa > 1:1911775/1‑70 (MQ=255) cacaATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTaa > 1:1866678/1‑70 (MQ=255) aTGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTTTCTCTTTAAAAAGa < 1:2109506/71‑1 (MQ=255) tGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGaa < 1:2177511/71‑1 (MQ=255) tGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGaa < 1:1408985/71‑1 (MQ=255) tGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGaa < 1:2749810/71‑1 (MQ=255) cTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgcagt < 1:2654643/38‑1 (MQ=255) gAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCg < 1:300169/71‑1 (MQ=255) gCTGGCGAGAAGGCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa < 1:2319999/60‑1 (MQ=255) gCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa < 1:2015452/60‑1 (MQ=255) gCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa < 1:3367439/60‑1 (MQ=255) gCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa < 1:421585/60‑1 (MQ=255) gCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa < 1:1818717/60‑1 (MQ=255) gCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTg < 1:908745/69‑1 (MQ=255) aTGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGaa < 1:1141936/47‑1 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa > 1:3305689/1‑43 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa > 1:3087077/1‑43 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa > 1:3079557/1‑43 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa > 1:21432/1‑43 (MQ=255) gCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGa > 1:1330820/1‑43 (MQ=255) aCAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGa < 1:972096/71‑1 (MQ=255) gggCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAc < 1:2754299/71‑1 (MQ=255) | TATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAC > W3110S.gb/1016227‑1016358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |