Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,020,867 | A→G | 100% | S159P (TCC→CCC) | sulA ← | SOS cell division inhibitor |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,020,867 | 0 | A | G | 76.5% | 29.0 / 5.6 | 17 | S159P (TCC→CCC) | sulA | SOS cell division inhibitor |
Reads supporting (aligned to +/- strand): ref base A (0/4); new base G (8/5); total (8/9) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.24e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAG > W3110S.gb/1020798‑1020923 | aCAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGaa < 1:1903148/71‑1 (MQ=255) aCAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGaa < 1:2282256/71‑1 (MQ=255) aaaaaaaGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAg < 1:3041643/71‑1 (MQ=255) aaaGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGt < 1:2551606/71‑1 (MQ=255) cAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTg < 1:2240448/70‑1 (MQ=255) aaaTTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAgg > 1:2231479/1‑67 (MQ=255) tACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCtt < 1:891058/70‑1 (MQ=255) aTACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCa < 1:2393545/71‑1 (MQ=255) aCAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCat > 1:3070548/1‑70 (MQ=255) cAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGa > 1:2080206/1‑51 (MQ=255) tGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:145375/63‑1 (MQ=255) tGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:1933156/63‑1 (MQ=255) tGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:3626713/63‑1 (MQ=255) tGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:213480/1‑70 (MQ=255) gAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGa > 1:908715/1‑53 (MQ=255) gAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGa > 1:1101261/1‑53 (MQ=255) gAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGa > 1:3679987/1‑53 (MQ=255) aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGagag < 1:1391928/37‑1 (MQ=255) aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg > 1:1902048/1‑71 (MQ=255) | ACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAG > W3110S.gb/1020798‑1020923 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |