| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,056,565 | G→A | 50.0% | G12G (GGC→GGT) | torS ← | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,056,565 | 0 | G | A | 50.0% | ‑1.5 / 32.8 | 26 | G12G (GGC→GGT) | torS | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
| Reads supporting (aligned to +/- strand): ref base G (4/9); new base A (8/5); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAG > W3110S.gb/1056508‑1056631 | cAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGc < 1:1156394/59‑1 (MQ=255)cAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGc < 1:926265/59‑1 (MQ=255)cAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGc < 1:3631449/59‑1 (MQ=255)cAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGc < 1:2866485/59‑1 (MQ=255) ggTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGccc > 1:1084478/1‑59 (MQ=255) ggTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGccc > 1:3180971/1‑59 (MQ=255) ggTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGccc > 1:2365626/1‑59 (MQ=255) tGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTc > 1:992873/1‑66 (MQ=255) tGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTc > 1:3648170/1‑66 (MQ=255) tGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTc > 1:2188266/1‑66 (MQ=255) tCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAggg > 1:2982243/1‑71 (MQ=255) caccaGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTa < 1:2408256/71‑1 (MQ=255) gggTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTc < 1:3122561/47‑1 (MQ=255) gTTAACAGCGCCATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACt > 1:2577019/1‑71 (MQ=255) ttAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTa < 1:1575338/51‑1 (MQ=255) ttAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTa < 1:281270/51‑1 (MQ=255) cATCAGGGCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGaaaa < 1:1902510/71‑1 (MQ=255) tCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGaaaa < 1:1467082/69‑1 (MQ=255) tCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGaaaa < 1:512975/69‑1 (MQ=255) tCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGaaaa < 1:1699109/69‑1 (MQ=255) aGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAg > 1:2405257/1‑69 (MQ=255) ggCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTaaa < 1:1996977/37‑1 (MQ=255) ggCAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTg < 1:1967065/71‑1 (MQ=255) cAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTGACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAg > 1:1937842/1‑71 (MQ=255) cAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAg > 1:386197/1‑71 (MQ=255) cAAAACCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAg > 1:564307/1‑71 (MQ=255) | CAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAG > W3110S.gb/1056508‑1056631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |