| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,075,828 | T→C | 66.7% | L1159L (TTA→TTG) | putA ← | fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,075,828 | 0 | T | C | 66.7% | 32.8 / 25.5 | 30 | L1159L (TTA→TTG) | putA | fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (5/5); new base C (15/5); total (20/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCT > W3110S.gb/1075759‑1075897 | gCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACAt < 1:3559882/70‑1 (MQ=255)gCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACAt < 1:2120838/70‑1 (MQ=255)gCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACAt < 1:1704587/70‑1 (MQ=255) cGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACAt < 1:2164027/69‑1 (MQ=255) gTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAAc < 1:2110229/71‑1 (MQ=255) ttCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGGCATATTGCGTACACAACg < 1:983128/71‑1 (MQ=255) cACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGcc > 1:1215555/1‑71 (MQ=255) cACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGcc > 1:230164/1‑71 (MQ=255) aCCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGcc < 1:1713114/70‑1 (MQ=255) caATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACaa > 1:3377784/1‑48 (MQ=255) caATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACaa > 1:2504925/1‑48 (MQ=255) caATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACaa > 1:2504239/1‑48 (MQ=255) aTCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGa > 1:1368819/1‑62 (MQ=255) gTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCaa > 1:1860726/1‑52 (MQ=255) ctagtgtTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGcc > 1:383094/4‑71 (MQ=255) tCGCCATATTGCGTACACAACGCCTGCAATTCTGGAc < 1:1501638/37‑1 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgc > 1:226565/1‑36 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgc > 1:3383781/1‑36 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgc > 1:2001642/1‑36 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgc > 1:2853708/1‑36 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgc > 1:2654127/1‑36 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc > 1:2585269/1‑70 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc > 1:3385600/1‑70 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc < 1:1238379/70‑1 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc < 1:541685/70‑1 (MQ=255) aTAACGCCTTCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCt > 1:1580655/1‑71 (MQ=255) aTAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCg < 1:260280/54‑1 (MQ=255) aTAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCg < 1:2486789/54‑1 (MQ=255) aTAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCg < 1:873419/54‑1 (MQ=255) aTAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCt > 1:2029988/1‑71 (MQ=255) aTAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCt > 1:2369947/1‑71 (MQ=255) aTAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCt > 1:2365784/1‑71 (MQ=255) aTAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCt < 1:1524407/71‑1 (MQ=255) aTAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCt > 1:1184841/1‑71 (MQ=255) | GCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCT > W3110S.gb/1075759‑1075897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |