| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,109,964 | G→A | 100% | C185C (TGC→TGT) | mdoC ← | membrane protein required for modification of periplasmic glucan |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,109,964 | 0 | G | A | 89.7% | 74.2 / 2.0 | 29 | C185C (TGC→TGT) | mdoC | membrane protein required for modification of periplasmic glucan |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (14/12); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAAT > W3110S.gb/1109901‑1110031 | aCTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaa > 1:1130721/1‑70 (MQ=255) cTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaaaa < 1:1406345/71‑1 (MQ=255) cTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaaaa < 1:2744982/71‑1 (MQ=255) tGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCAcc > 1:223422/1‑71 (MQ=255) ggCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaa > 1:981132/1‑60 (MQ=255) ggCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaa > 1:1046097/1‑60 (MQ=255) ggCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaa > 1:899379/1‑60 (MQ=255) gATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGt < 1:678578/71‑1 (MQ=255) tACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGttt > 1:3569026/1‑71 (MQ=255) tACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGttt > 1:1443385/1‑71 (MQ=255) cacaATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaaaaaa > 1:2525387/1‑56 (MQ=255) caATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGtttt < 1:1991795/68‑1 (MQ=255) caATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGtttt < 1:3045083/68‑1 (MQ=255) caATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGtttt < 1:1437656/68‑1 (MQ=255) caATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGtttt < 1:2395103/68‑1 (MQ=255) caATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGtttt < 1:2650328/68‑1 (MQ=255) aTAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCAt > 1:2626069/1‑71 (MQ=255) gTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGt > 1:1611871/1‑54 (MQ=255) gTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGt > 1:2167445/1‑54 (MQ=255) tcttctTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAAttttt > 1:674573/1‑71 (MQ=255) cATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTAt > 1:2554754/1‑69 (MQ=255) tAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTc < 1:146429/60‑1 (MQ=255) aCCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAAt > 1:1217967/1‑71 (MQ=255) aCCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTAtt > 1:1377930/1‑57 (MQ=255) aCCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAAt > 1:3607237/1‑71 (MQ=255) aCCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAAt > 1:1680281/1‑71 (MQ=255) ccGAGACATAAAAATATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTaa < 1:2389447/71‑1 (MQ=255) ccGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTaa < 1:1806980/71‑1 (MQ=255) ccGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTaa < 1:3633732/71‑1 (MQ=255) gagaCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAat > 1:2100308/1‑71 (MQ=255) gagaCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAat > 1:3569761/1‑71 (MQ=255) gagaCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAat > 1:1726776/1‑71 (MQ=255) | ACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAAT > W3110S.gb/1109901‑1110031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |