| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,172,268 | T→C | 100% | K1092E (AAG→GAG) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,172,268 | 0 | T | C | 100.0% | 73.5 / NA | 22 | K1092E (AAG→GAG) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/11); total (11/11) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCT > W3110S.gb/1172203‑1172312 | aaGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTctcctc > 1:1594541/1‑69 (MQ=255) gCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCaa < 1:2486664/71‑1 (MQ=255) tgctgcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt < 1:3372788/71‑1 (MQ=255) ctgcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:1612079/1‑71 (MQ=255) ctgcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:954182/1‑71 (MQ=255) gTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCaaa > 1:3007076/1‑55 (MQ=255) gTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCaaa > 1:3516321/1‑55 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGa > 1:2135403/1‑57 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGa > 1:2930014/1‑57 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGa > 1:3096908/1‑57 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTtc > 1:3433078/1‑71 (MQ=255) tGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTtct < 1:356452/70‑1 (MQ=255) agcaAACCAATGAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCAtt < 1:355067/71‑1 (MQ=255) ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:2712009/1‑49 (MQ=255) ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:3153984/1‑49 (MQ=255) cAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTcc < 1:3130383/71‑1 (MQ=255) cAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTcc < 1:3032443/71‑1 (MQ=255) aaTCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTc > 1:3596116/1‑69 (MQ=255) aaCCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCt < 1:3165264/70‑1 (MQ=255) aaCCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCt < 1:2793267/70‑1 (MQ=255) ccAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt < 1:277478/39‑1 (MQ=255) ccAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt < 1:550058/39‑1 (MQ=255) ccAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt < 1:250780/39‑1 (MQ=255) ttctccTCGGCAAATTCGATCACCCCGCCTTtctc < 1:2192518/35‑1 (MQ=255) ttctccTCGGCAAATTCGATCACCCCGCCTTtctc < 1:3682896/35‑1 (MQ=255) | AAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCT > W3110S.gb/1172203‑1172312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |