| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,173,170 | C→T | 100% | R791H (CGT→CAT) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,173,170 | 0 | C | T | 90.5% | 53.1 / ‑0.6 | 21 | R791H (CGT→CAT) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (7/12); total (9/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGC > W3110S.gb/1173112‑1173208 | tcctccGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAgg < 1:722057/68‑1 (MQ=255) ctccGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGtttt > 1:1167565/1‑70 (MQ=255) ctccGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGtttt > 1:3490068/1‑70 (MQ=255) aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTTCCAgacg < 1:659283/71‑1 (MQ=255) aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacg < 1:1118417/71‑1 (MQ=255) aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacg < 1:920077/71‑1 (MQ=255) aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacg < 1:2567042/71‑1 (MQ=255) tttCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACaa < 1:2128381/51‑1 (MQ=255) tttCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACaa < 1:464049/51‑1 (MQ=255) tttCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGtttt < 1:3274673/58‑1 (MQ=255) tCGCCTCCCGGACCACCATGCTGTCATACTCATGGACaaa > 1:1988758/1‑40 (MQ=255) tCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg > 1:2447909/1‑41 (MQ=255) tCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg > 1:1126395/1‑41 (MQ=255) ccGGACCACCATGCTGTCATACTCATGGACAAAGGtttt < 1:1775793/39‑1 (MQ=255) ccGGACCACCATGCTGTCATACTCATGGACAAAGGtttt < 1:1643239/39‑1 (MQ=255) ccGGACCACCATGCTGTCATACTCATGGACAAAGGtttt < 1:2742382/39‑1 (MQ=255) ccGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgg > 1:1034176/1‑55 (MQ=255) cTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacg < 1:1299885/38‑1 (MQ=255) cATACTCATGGACAAAGGTTTTAACTGCCAGACGACGGGCGggcggc > 1:1728199/1‑47 (MQ=255) cATACTCATGGACAAAGGTTTTAACTGCCAGACGACGGGCGggcggc > 1:636074/1‑47 (MQ=255) cATACTCATGGACAAAGGTTTTAACTGCCAGACGACGGGCGggcggc > 1:1270360/1‑47 (MQ=255) | TCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGC > W3110S.gb/1173112‑1173208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |