| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,175,236 | C→A | 100% | Q102H (CAG→CAT) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,175,236 | 0 | C | A | 92.0% | 59.9 / 0.9 | 25 | Q102H (CAG→CAT) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (4/19); total (6/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAA > W3110S.gb/1175175‑1175300 | gaAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCAtcgtc < 1:3360911/71‑1 (MQ=255)gaAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCAtcgtc < 1:2743038/71‑1 (MQ=255)gaAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCAtcgtc < 1:2379698/71‑1 (MQ=255) aaaaCTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCAtcgtcg > 1:601715/1‑71 (MQ=255) aaaaCTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCAtcgtcg > 1:604079/1‑71 (MQ=255) gCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaa < 1:733602/70‑1 (MQ=255) gCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaa < 1:3667491/70‑1 (MQ=255) gCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaa < 1:1620357/70‑1 (MQ=255) cATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGgc > 1:1739520/1‑71 (MQ=255) tAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTATCTGGTaaa < 1:2374430/57‑1 (MQ=255) tAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaaa < 1:328729/57‑1 (MQ=255) tAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaaa < 1:365829/57‑1 (MQ=255) tAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaaa < 1:359068/57‑1 (MQ=255) ttCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGAt < 1:161196/71‑1 (MQ=255) ttCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGAt < 1:720621/71‑1 (MQ=255) ttCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGAt < 1:349000/71‑1 (MQ=255) tCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGAt < 1:1956804/70‑1 (MQ=255) aaCAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCt < 1:1445683/71‑1 (MQ=255) aGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgag > 1:2988253/1‑51 (MQ=255) aGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgag > 1:1491652/1‑51 (MQ=255) aGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgag > 1:1052518/1‑51 (MQ=255) gCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGcgcg < 1:344166/44‑1 (MQ=255) gCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGcgcg < 1:3694952/44‑1 (MQ=255) gCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGcgcg < 1:2564996/44‑1 (MQ=255) gCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGaaa < 1:3171636/71‑1 (MQ=255) | GAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAA > W3110S.gb/1175175‑1175300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |