| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,181,288 | T→G | 100% | D64E (GAT→GAG) | cobB → | deacetylase of acetyl‑CoA synthetase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,181,288 | 0 | T | G | 78.4% | 97.9 / 12.9 | 37 | D64E (GAT→GAG) | cobB | deacetylase of acetyl‑CoA synthetase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (7/1); new base G (21/8); total (28/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGATCCTG > W3110S.gb/1181226‑1181352 | cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCtgtg > 1:1387818/1‑71 (MQ=255)cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCtgtg > 1:3454985/1‑71 (MQ=255)cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCtgt > 1:3458420/1‑70 (MQ=255)cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCtgtg > 1:411800/1‑71 (MQ=255)cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCtgtg > 1:2782875/1‑71 (MQ=255) gTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTggg > 1:2001623/1‑70 (MQ=255) gTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTggg > 1:2421410/1‑70 (MQ=255) ttCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGAt < 1:1920874/70‑1 (MQ=255) aTTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAgg > 1:125046/1‑71 (MQ=255) aTTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAgg > 1:39556/1‑71 (MQ=255) aTTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAgg > 1:3204945/1‑71 (MQ=255) aTTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAgg > 1:2621154/1‑71 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGaa < 1:691508/50‑1 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGaa < 1:218869/50‑1 (MQ=255) cGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATc > 1:2992448/1‑40 (MQ=255) cGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGa > 1:2800371/1‑50 (MQ=255) cGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTgg > 1:2945513/1‑55 (MQ=255) cGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGttt > 1:2430854/1‑71 (MQ=255) cGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGttt > 1:2448203/1‑71 (MQ=255) cGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGttt > 1:3209332/1‑71 (MQ=255) cGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGtt > 1:220122/1‑70 (MQ=255) tttCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGa > 1:2355138/1‑44 (MQ=255) tttCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGa > 1:2556735/1‑44 (MQ=255) tttCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGa > 1:2197432/1‑44 (MQ=255) ttCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGAtg > 1:2387452/1‑45 (MQ=255) ttCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGAtg > 1:1485646/1‑45 (MQ=255) ttCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGAtg > 1:1674290/1‑45 (MQ=255) ttCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGAtg > 1:2900859/1‑45 (MQ=255) ttCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTgg > 1:622608/1‑48 (MQ=255) ttCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTgg > 1:1217654/1‑48 (MQ=255) tCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATcgc > 1:342199/1‑70 (MQ=255) cgcgCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATcgcg < 1:1971484/70‑1 (MQ=255) cgcgCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATcgcg < 1:1403581/70‑1 (MQ=255) gcgcCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGa < 1:309512/70‑1 (MQ=255) gcgcCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGa < 1:1834449/70‑1 (MQ=255) ccgcAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCC‑GAAGGTTTCGATCGCGATCCTg < 1:2866506/71‑1 (MQ=255) cgcAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGATCCTg < 1:3437475/71‑1 (MQ=255) | CTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGATCCTG > W3110S.gb/1181226‑1181352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |