| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,185,915 | A→G | 48.8% | M36T (ATG→ACG) | potB ← | polyamine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,185,915 | 0 | A | G | 48.8% | ‑5.3 / 59.7 | 41 | M36T (ATG→ACG) | potB | polyamine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (7/14); new base G (16/4); total (23/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
CAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGA > W3110S.gb/1185854‑1185984 | cAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa < 1:2584153/71‑1 (MQ=255)cAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa < 1:3180569/71‑1 (MQ=255)cAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa < 1:3660795/71‑1 (MQ=255) tAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACAAAAc > 1:2081059/1‑71 (MQ=255) aGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACAAAACt > 1:1270946/1‑71 (MQ=255) aGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACAAAACt > 1:1553967/1‑71 (MQ=255) aGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACt > 1:2243479/1‑71 (MQ=255) aCTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGc < 1:2105542/71‑1 (MQ=255) agagCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCgg > 1:1650340/1‑71 (MQ=255) aTCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCaaaa < 1:2512613/71‑1 (MQ=255) tCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCaaa > 1:2375983/1‑69 (MQ=255) gACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGGGTCGTCGCGGGTCAAAAAGCTg < 1:1593717/68‑1 (MQ=255) gACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTg < 1:401774/68‑1 (MQ=255) cgcgTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGc > 1:2104195/1‑70 (MQ=255) tAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat > 1:34202/1‑71 (MQ=255) tAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGa > 1:2704446/1‑70 (MQ=255) tAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:925892/71‑1 (MQ=255) tAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:2649060/71‑1 (MQ=255) aGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:1483042/70‑1 (MQ=255) tATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatcat > 1:2316298/1‑70 (MQ=255) tATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatcat > 1:3591728/1‑70 (MQ=255) tATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatcat > 1:3306076/1‑70 (MQ=255) aTCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatcat > 1:3164238/1‑69 (MQ=255) tCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCGGGTGCCAATGAtcatca < 1:183280/70‑1 (MQ=255) gCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCATGTTg > 1:2140386/1‑71 (MQ=255) tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCaaaaa > 1:2378096/1‑43 (MQ=255) tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCaaaaa > 1:841027/1‑43 (MQ=255) tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCaaaaa > 1:3229746/1‑43 (MQ=255) tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCaaaaa > 1:281681/1‑43 (MQ=255) tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:2864198/58‑1 (MQ=255) tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:2779319/58‑1 (MQ=255) aaGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatca > 1:1152492/1‑58 (MQ=255) aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGTCAATGATCATCAGGTTggg > 1:1220071/1‑68 (MQ=255) aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTggg > 1:3357043/1‑68 (MQ=255) aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTggg > 1:3593840/1‑68 (MQ=255) aCCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGa < 1:3107109/69‑1 (MQ=255) ccATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGa > 1:2583777/1‑68 (MQ=255) cATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGa < 1:198862/71‑1 (MQ=255) cATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGa < 1:3430059/71‑1 (MQ=255) cATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGa < 1:347191/71‑1 (MQ=255) cATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGa < 1:72756/71‑1 (MQ=255) | CAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGA > W3110S.gb/1185854‑1185984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |