| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,244,320 | C→T | 100% | C113Y (TGC→TAC) | ldcA ← | L,D‑carboxypeptidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,244,320 | 0 | C | T | 91.8% | 170.2 / 6.4 | 61 | C113Y (TGC→TAC) | ldcA | L,D‑carboxypeptidase A |
| Reads supporting (aligned to +/- strand): ref base C (0/5); new base T (30/26); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACC > W3110S.gb/1244254‑1244385 | gcgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg < 1:411612/71‑1 (MQ=255)gcgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg < 1:3399783/71‑1 (MQ=255)gcgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg < 1:2267902/71‑1 (MQ=255)gcgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg < 1:2125414/71‑1 (MQ=255)gcgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg < 1:1981434/71‑1 (MQ=255)gcgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg < 1:3672018/71‑1 (MQ=255)gcgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg < 1:3269930/71‑1 (MQ=255)gcgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg < 1:30188/71‑1 (MQ=255) tttGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCgg > 1:1088640/1‑70 (MQ=255) tttGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCgg > 1:1980139/1‑70 (MQ=255) cTACCAGCATGGGGCCGCTAAATGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTaaa < 1:1620467/70‑1 (MQ=255) cTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTaaa < 1:1013334/70‑1 (MQ=255) cTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTaaa < 1:1247894/70‑1 (MQ=255) cTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTaaa < 1:736827/70‑1 (MQ=255) cTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTaaa < 1:2662691/70‑1 (MQ=255) cTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTaaa < 1:1217064/70‑1 (MQ=255) cAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGc < 1:339087/71‑1 (MQ=255) cAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGc < 1:812112/71‑1 (MQ=255) aGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGc > 1:2940024/1‑70 (MQ=255) aGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGc > 1:2874412/1‑70 (MQ=255) tGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGa < 1:392768/50‑1 (MQ=255) tGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGa < 1:3660028/50‑1 (MQ=255) tGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGa < 1:2412339/50‑1 (MQ=255) tGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTg < 1:2405618/49‑1 (MQ=255) tGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTg < 1:1959201/49‑1 (MQ=255) tGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTg < 1:1955603/49‑1 (MQ=255) ggggCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTAc > 1:1182343/1‑46 (MQ=255) ggggCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTAc > 1:3113428/1‑46 (MQ=255) ggggCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTg < 1:3381376/48‑1 (MQ=255) gggCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAAt > 1:2335890/1‑50 (MQ=255) gggCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAAt > 1:3222052/1‑50 (MQ=255) gggCAGATAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAAt > 1:476715/1‑50 (MQ=255) aaaGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGaa > 1:805132/1‑41 (MQ=255) aaaGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGaa > 1:3292606/1‑41 (MQ=255) aaaGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGaa > 1:3300599/1‑41 (MQ=255) aaGGTGATGACATTGCCATGCGCCAGAAGACCGTAc > 1:2891630/1‑36 (MQ=255) aaGGTGATGACATTGCCATGCGCCAGAAGACCGTAc > 1:1920858/1‑36 (MQ=255) aaGGTGATGACATTGCCATGCGCCAGAAGACCGTAc > 1:3693827/1‑36 (MQ=255) aaGGTGATGACATTGCCATGCGCCAGAAGACCGTAc > 1:774022/1‑36 (MQ=255) aaGGTGATGACATTGCCATGCGCCAGAAGACCGTAc > 1:2226182/1‑36 (MQ=255) aaGGTGATGACATTGCCATGCGCCAGAAGACCGTAc > 1:448235/1‑36 (MQ=255) aaGGTGATGACATTGCCATGCGCCAGAAGACCGTAc > 1:1289255/1‑36 (MQ=255) aTTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAt < 1:1686399/71‑1 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt > 1:2790522/1‑71 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt > 1:781033/1‑71 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt > 1:344085/1‑71 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt > 1:3419163/1‑71 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt > 1:168871/1‑71 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgt > 1:2188923/1‑70 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtg > 1:1840671/1‑69 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtg > 1:126326/1‑69 (MQ=255) ccATGCGCCAGAAGACCGTACTGAATGGCGTTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt < 1:2707276/70‑1 (MQ=255) cATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgttgt < 1:2785725/71‑1 (MQ=255) cgcCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGc > 1:2499610/1‑71 (MQ=255) cgcCAGAAGACCGTACTGAATGGCGGTAAAATAGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGc > 1:957606/1‑71 (MQ=255) agaagaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGc < 1:2917691/71‑1 (MQ=255) gaagaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGc > 1:352031/1‑70 (MQ=255) gaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccacc < 1:2415996/71‑1 (MQ=255) gaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccacc < 1:1984507/71‑1 (MQ=255) gaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccacc < 1:819049/71‑1 (MQ=255) aCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccacc < 1:2463131/70‑1 (MQ=255) | GCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACC > W3110S.gb/1244254‑1244385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |