Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,633 G→T 100% Q246Y (CAG→TAT oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6330GT100.0% 98.0 / NA 33Q246Y (CAG→TAToppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/19);  total (14/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTG  >  W3110S.gb/1303568‑1303688
                                                                 |                                                       
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                     >  1:1873899/1‑70 (MQ=255)
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                    >  1:206286/1‑71 (MQ=255)
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                    >  1:2203028/1‑71 (MQ=255)
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                    >  1:2712420/1‑71 (MQ=255)
     acgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                               <  1:2834178/71‑1 (MQ=255)
         aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                           <  1:3593853/71‑1 (MQ=255)
          tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                >  1:488511/1‑65 (MQ=255)
          tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                >  1:1230658/1‑65 (MQ=255)
          tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                >  1:1909011/1‑65 (MQ=255)
               cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTg                                      <  1:430754/70‑1 (MQ=255)
               cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTg                                      <  1:3201499/70‑1 (MQ=255)
                 tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                   >  1:626922/1‑71 (MQ=255)
                 tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                   >  1:1821091/1‑71 (MQ=255)
                    aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                <  1:3092916/71‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                  <  1:2288096/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                  <  1:3610861/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                  <  1:3604281/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                  <  1:3496116/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                  <  1:1584055/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                  <  1:2799481/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                  <  1:2795544/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                  <  1:2724045/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                  <  1:2509943/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa                 <  1:1165351/71‑1 (MQ=255)
                                          aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                              >  1:2229315/1‑51 (MQ=255)
                                          aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                              >  1:2271573/1‑51 (MQ=255)
                                          aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                              >  1:1229059/1‑51 (MQ=255)
                                          aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTATGaa                              >  1:2899742/1‑51 (MQ=255)
                                           acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAc         <  1:3113454/71‑1 (MQ=255)
                                           acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAc         <  1:1050689/71‑1 (MQ=255)
                                              cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt      <  1:1468228/71‑1 (MQ=255)
                                               gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt      <  1:3320504/70‑1 (MQ=255)
                                                  aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtg  >  1:2600153/1‑71 (MQ=255)
                                                                 |                                                       
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTG  >  W3110S.gb/1303568‑1303688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: