| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,339,708 | G→A | 100% | A722T (GCC→ACC) | acnA → | aconitate hydratase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,339,708 | 0 | G | A | 97.1% | 95.9 / ‑3.8 | 34 | A722T (GCC→ACC) | acnA | aconitate hydratase 1 |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (14/19); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGAAAAGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCGCCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCTGA > W3110S.gb/1339642‑1339775 | cGAAAAGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCaa < 1:2710489/71‑1 (MQ=255)cGAAAAGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCaa < 1:1618044/71‑1 (MQ=255) aGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTc > 1:3216805/1‑71 (MQ=255) aGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTc > 1:3580480/1‑71 (MQ=255) gACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCg < 1:1137597/71‑1 (MQ=255) gACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCg < 1:2637956/71‑1 (MQ=255) gACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCg < 1:2336718/71‑1 (MQ=255) aCTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCg > 1:1581356/1‑70 (MQ=255) cGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGaaa > 1:1855207/1‑71 (MQ=255) cGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGaaa > 1:1739409/1‑71 (MQ=255) tCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGt > 1:2044441/1‑71 (MQ=255) cgcgGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGt < 1:559070/70‑1 (MQ=255) cgcgGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGt < 1:2238071/70‑1 (MQ=255) gTAACCATGAAGTGATGATGCGCGGCACCTTCGCCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGt < 1:487627/70‑1 (MQ=255) gTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGtt > 1:2785389/1‑71 (MQ=255) gTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGtt > 1:1150736/1‑71 (MQ=255) tAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCTCATCCGTAATGAAATGGTGCCTGGCg < 1:2069329/68‑1 (MQ=255) tAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGaa < 1:3523039/55‑1 (MQ=255) tAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGaa < 1:3518682/55‑1 (MQ=255) tAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGaa < 1:919780/55‑1 (MQ=255) tAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCg < 1:24826/68‑1 (MQ=255) tAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTg < 1:2425121/71‑1 (MQ=255) tAACCATGAAGTGATGAAGCGCGGCACCTTCACCaa < 1:407838/36‑1 (MQ=37) aaCCATGAAGTGATGATGCGCGGCACCTTCACCaa < 1:3597736/35‑1 (MQ=255) aCCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTg > 1:2568674/1‑69 (MQ=255) aCCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTg > 1:2841060/1‑69 (MQ=255) aCCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGaa < 1:2024293/71‑1 (MQ=255) aCCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGaa < 1:1058855/71‑1 (MQ=255) aCCATGAAGTGATGATGCGCGGCACCTTCACC‑ATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGaa < 1:430657/70‑1 (MQ=255) gatgatGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGa > 1:2803422/1‑71 (MQ=255) gatgatGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGa > 1:566859/1‑71 (MQ=255) ggCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCAttt < 1:2500488/71‑1 (MQ=255) ttCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCTGa > 1:2151860/1‑71 (MQ=255) ttCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCTGa > 1:1926968/1‑71 (MQ=255) | CGAAAAGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCGCCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCTGA > W3110S.gb/1339642‑1339775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |