| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,373,368 | G→A | 86.3% | R480H (CGT→CAT) | ycjM → | predicted glucosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,373,368 | 0 | G | A | 86.3% | 204.9 / 23.2 | 80 | R480H (CGT→CAT) | ycjM | predicted glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (9/2); new base A (38/31); total (47/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
AACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGT > W3110S.gb/1373302‑1373431 | aaCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:2367918/1‑70 (MQ=255)aaCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:2363746/1‑70 (MQ=255) aTGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc < 1:18886/71‑1 (MQ=255) aTGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc < 1:815806/71‑1 (MQ=255) aTGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc < 1:1781461/71‑1 (MQ=255) aTGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc < 1:1388317/71‑1 (MQ=255) tGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACg < 1:983694/71‑1 (MQ=255) gAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGACATAACg < 1:1066540/70‑1 (MQ=255) ttAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATcc > 1:607099/1‑71 (MQ=255) aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCg > 1:524632/1‑70 (MQ=255) aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCg > 1:3637682/1‑70 (MQ=255) aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCg > 1:3584655/1‑70 (MQ=255) aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCg > 1:2754425/1‑70 (MQ=255) tataTCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATACtttt < 1:1628087/71‑1 (MQ=255) tataTCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt < 1:148556/71‑1 (MQ=255) tataTCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt < 1:1632472/71‑1 (MQ=255) tatCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACg < 1:929643/46‑1 (MQ=255) tatCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAc < 1:2484503/71‑1 (MQ=255) tatCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGACTTTCATCCGGATAATAATTTTAc > 1:1588769/1‑71 (MQ=255) atCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAc < 1:3648689/70‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCa < 1:2271483/51‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:268263/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1925070/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCCGCCATAACGAGTTTCATCCGGATAATAATTTTAcc > 1:3279049/1‑70 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAttt > 1:3568652/1‑66 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:1924129/70‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:1382268/70‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc > 1:841482/1‑70 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:3532695/70‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:3225411/70‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:923734/70‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:928044/70‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:2969095/70‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:2757169/70‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1521955/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1210470/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:583650/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa < 1:117364/71‑1 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1387663/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1952742/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:145296/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1982731/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:3285400/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:3241495/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:3012091/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:2529208/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:2974575/1‑71 (MQ=255) tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:2621248/1‑71 (MQ=255) aTGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAt > 1:3012686/1‑70 (MQ=255) aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt > 1:36361/1‑71 (MQ=255) aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt > 1:2672668/1‑71 (MQ=255) aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt > 1:541294/1‑71 (MQ=255) aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAt > 1:3162278/1‑70 (MQ=255) ttGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:2598273/66‑1 (MQ=255) ttGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:265238/66‑1 (MQ=255) ttGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:3656146/66‑1 (MQ=255) ttGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:447952/66‑1 (MQ=255) gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGa > 1:735006/1‑71 (MQ=255) gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGa > 1:737899/1‑71 (MQ=255) cgcgTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:3446528/1‑53 (MQ=255) cgcgTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:2267201/1‑53 (MQ=255) cgcgTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:934357/1‑53 (MQ=255) gcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGAtt < 1:1021863/70‑1 (MQ=255) gcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGAtt > 1:361537/1‑70 (MQ=255) gcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGAtt < 1:3290753/70‑1 (MQ=255) gTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAt < 1:2416610/71‑1 (MQ=255) tttAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGat < 1:2422629/43‑1 (MQ=255) tAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa > 1:3412000/1‑71 (MQ=255) tAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa > 1:1499700/1‑71 (MQ=255) tAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa > 1:1557615/1‑71 (MQ=255) tAATTACACTTCATCGCAGCCATAACGAGTTTCAt > 1:2253832/1‑35 (MQ=255) tAATTACACTTCATCGCAGCCATAACGAGTTTCAt > 1:967426/1‑35 (MQ=255) tAATTACACTTCATCGCAGCCATAACGAGTTTCAt > 1:1103474/1‑35 (MQ=255) tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:1026658/1‑43 (MQ=255) tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:450894/1‑43 (MQ=255) tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:16049/1‑43 (MQ=255) tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:2331512/1‑43 (MQ=255) tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:1901521/1‑43 (MQ=255) tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa > 1:1408886/1‑71 (MQ=255) cacTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:315089/70‑1 (MQ=255) cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:3154501/70‑1 (MQ=255) cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:184169/70‑1 (MQ=255) | AACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGT > W3110S.gb/1373302‑1373431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |