Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,376,428 G→A 100% V209M (GTG→ATG)  ycjP → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,376,4280GA89.3% 72.9 / 0.9 28V209M (GTG→ATG) ycjPpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (13/12);  total (15/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCTTACCGG  >  W3110S.gb/1376374‑1376494
                                                      |                                                                  
aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACt                                                     >  1:990916/1‑70 (MQ=255)
aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTa                                                    >  1:2649947/1‑71 (MQ=255)
aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGa                                                       >  1:3176767/1‑68 (MQ=255)
aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTa                                                    >  1:1397915/1‑71 (MQ=255)
aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTa                                                    >  1:1571363/1‑71 (MQ=255)
aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTa                                                    >  1:2636186/1‑71 (MQ=255)
aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTa                                                    >  1:3476396/1‑71 (MQ=255)
aCCGTGCCGTTGCCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGa                                                       >  1:3618774/1‑68 (MQ=255)
      ccGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGt                                               <  1:321536/70‑1 (MQ=255)
              gATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGa                                      <  1:3511779/71‑1 (MQ=255)
              gATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGa                                      <  1:2368318/71‑1 (MQ=255)
                  tCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGtt                                              <  1:1205701/59‑1 (MQ=255)
                  tCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGtt                                              <  1:1879337/59‑1 (MQ=255)
                  tCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGtt                                              <  1:1884570/59‑1 (MQ=255)
                  tCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGtt                                              <  1:2025099/59‑1 (MQ=255)
                    cGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTcc                                >  1:2173732/1‑71 (MQ=255)
                            tatCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAgc                        >  1:3417437/1‑71 (MQ=255)
                            tatCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAg                         >  1:1451291/1‑70 (MQ=255)
                                      cGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGAtt                                    >  1:1001520/1‑49 (MQ=255)
                                      cGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAAtt              >  1:3198719/1‑71 (MQ=255)
                                           aCTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGc                  <  1:2828633/62‑1 (MQ=255)
                                           aCTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGc                  <  1:1641949/62‑1 (MQ=255)
                                            cTGCTTTATGGTGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCa           <  1:2865184/68‑1 (MQ=255)
                                               cTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCTTa      <  1:2780881/70‑1 (MQ=255)
                                               cTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCTTa      <  1:987761/70‑1 (MQ=255)
                                               cTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTACAGCGCCAGCAATTTCACCTTa      <  1:1649970/70‑1 (MQ=255)
                                                  tATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCTTACCgg  >  1:1776768/1‑71 (MQ=255)
                                                  tATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCTTAACgg  >  1:961165/1‑71 (MQ=255)
                                                      |                                                                  
ACCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCTTACCGG  >  W3110S.gb/1376374‑1376494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑