| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,426,674 | T→C | 83.8% | D393D (GAT→GAC) | trkG → | potassium transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,426,674 | 0 | T | C | 83.8% | 91.3 / 10.3 | 37 | D393D (GAT→GAC) | trkG | potassium transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base C (11/20); total (13/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTATACTGG > W3110S.gb/1426610‑1426741 | tAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:3005085/70‑1 (MQ=255)tAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:2462310/70‑1 (MQ=255) aaTCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAAt < 1:2032960/71‑1 (MQ=255) aaTCAGCCTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGAAAGATAGTTACAGACCGTGTAAt < 1:1048721/71‑1 (MQ=255) aGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAg < 1:724580/70‑1 (MQ=255) cTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCgtgt < 1:1640301/62‑1 (MQ=255) cTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCgtgt < 1:696308/62‑1 (MQ=255) cTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCgtgt < 1:3403519/62‑1 (MQ=255) ctcATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGt < 1:1054012/70‑1 (MQ=255) aTCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTa < 1:3062956/68‑1 (MQ=255) aTCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTa < 1:767113/68‑1 (MQ=255) aTCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTa < 1:548423/68‑1 (MQ=255) aTCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTa < 1:3688355/68‑1 (MQ=255) aTCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTa < 1:2764229/68‑1 (MQ=255) aTCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTa < 1:2091710/68‑1 (MQ=255) ccAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGt > 1:3091345/1‑71 (MQ=255) agCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttct < 1:2112862/71‑1 (MQ=255) gCGTTGTTGAGTGTAAATATAGGAGGGAAGATAGTTACAGACCgtg > 1:761106/1‑46 (MQ=255) gAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttcttttttctt < 1:567262/70‑1 (MQ=255) gAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttcttttttctt < 1:3264448/70‑1 (MQ=255) tAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATgg < 1:2775825/50‑1 (MQ=255) tAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATgg < 1:3478115/50‑1 (MQ=255) tAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttcttttttctt < 1:3082732/65‑1 (MQ=255) tAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAct > 1:1619470/1‑71 (MQ=255) aaaTGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATActc > 1:1025968/1‑71 (MQ=255) aaaTGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAct > 1:3703593/1‑70 (MQ=255) aaTGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATActc > 1:879724/1‑70 (MQ=255) tAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCtt > 1:3310776/1‑70 (MQ=255) tAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCtt > 1:198769/1‑70 (MQ=255) aTAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt < 1:2394174/71‑1 (MQ=255) aTAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt > 1:2095686/1‑71 (MQ=255) aGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta > 1:3243164/1‑71 (MQ=255) aCAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTATACt > 1:3658021/1‑71 (MQ=255) aCAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTATACt > 1:2518092/1‑71 (MQ=255) aCAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTATACt > 1:1340011/1‑71 (MQ=255) aGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTATACTgg < 1:3038515/71‑1 (MQ=255) aGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTATACTgg < 1:899078/71‑1 (MQ=255) | TAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTATACTGG > W3110S.gb/1426610‑1426741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |