| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,441,198 | G→A | 17.4% | T434I (ACC→ATC) | ydbK ← | fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,441,198 | 0 | G | A | 17.4% | 110.9 / 15.8 | 46 | T434I (ACC→ATC) | ydbK | fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (23/15); new base A (6/2); total (29/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAAACAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAGTTTGGC > W3110S.gb/1441134‑1441263 | gTAAACAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTcgcg > 1:1741520/1‑70 (MQ=255)gTAAACAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTcgcg > 1:2657621/1‑70 (MQ=255) aaaTAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCt < 1:1763599/71‑1 (MQ=255) aaaTAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCt < 1:2152887/71‑1 (MQ=255) aTAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc < 1:166481/71‑1 (MQ=255) gCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCAt < 1:902270/71‑1 (MQ=255) gCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCAt < 1:1947660/71‑1 (MQ=255) cccTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGa < 1:3020451/59‑1 (MQ=255) cccTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATc < 1:1213869/71‑1 (MQ=255) ccTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATc < 1:1081929/70‑1 (MQ=255) cGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTAcc > 1:474933/1‑70 (MQ=255) cGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTAcc > 1:2744808/1‑70 (MQ=255) ccACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCAt < 1:910975/59‑1 (MQ=255) ccACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCAt < 1:848378/59‑1 (MQ=255) ccACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCAt < 1:2811008/59‑1 (MQ=255) ccACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCAt < 1:251551/59‑1 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCa > 1:1519540/1‑59 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCa > 1:1240610/1‑59 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCa > 1:3254586/1‑59 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCa > 1:1937649/1‑59 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCa > 1:2712047/1‑59 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCa > 1:1931411/1‑59 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCa > 1:2064465/1‑59 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCa > 1:1818652/1‑71 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCa > 1:2112159/1‑71 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCa > 1:857168/1‑71 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCa > 1:3595489/1‑71 (MQ=255) aCCGATAATCTTGATATTGTTTTTGGTCGCGGaaa > 1:2423342/1‑35 (MQ=255) aCCGATAATCTTGATATTGTTTTTGGTCGCGGaaa > 1:132787/1‑35 (MQ=255) aCCGATAATCTTGATATTGTTTTTGGTCGCGGaaa > 1:636403/1‑35 (MQ=255) aCCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCtt > 1:590494/1‑71 (MQ=255) aCCGATAATCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCtt > 1:2294090/1‑71 (MQ=255) aCCGATAATCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCtt > 1:1826227/1‑71 (MQ=255) aCCGATAATCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCtt > 1:3551319/1‑71 (MQ=255) aaTCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGtt > 1:2138349/1‑71 (MQ=255) aTCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGttt > 1:197141/1‑71 (MQ=255) aTCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGttt > 1:3317269/1‑71 (MQ=255) aTCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGttt > 1:210552/1‑71 (MQ=255) atatTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGa < 1:3595470/71‑1 (MQ=255) tatTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGa < 1:1591307/70‑1 (MQ=255) ttGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTcc < 1:3334672/57‑1 (MQ=255) ttGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTcc < 1:2419837/57‑1 (MQ=255) gTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATaaaa > 1:1360453/1‑45 (MQ=255) gTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATaaaa > 1:1755053/1‑45 (MQ=255) gTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAGTTTg > 1:776558/1‑71 (MQ=255) tttGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAGTTTGGc < 1:1242982/70‑1 (MQ=255) | GTAAACAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAGTTTGGC > W3110S.gb/1441134‑1441263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |