| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,463,502 | C→T | 100% | P299L (CCG→CTG) | paaJ → | predicted beta‑ketoadipyl CoA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,463,502 | 0 | C | T | 100.0% | 80.4 / NA | 27 | P299L (CCG→CTG) | paaJ | predicted beta‑ketoadipyl CoA thiolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/12); total (15/12) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATTGA > W3110S.gb/1463440‑1463568 | gcgGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTg > 1:1586372/1‑71 (MQ=255)gcgGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTg > 1:508868/1‑71 (MQ=255)gcgGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTg > 1:3511807/1‑71 (MQ=255) tCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCgg > 1:1497944/1‑71 (MQ=255) gTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGt > 1:949947/1‑71 (MQ=255) cATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTgg < 1:1631402/71‑1 (MQ=255) cAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAAcgc > 1:1836162/1‑71 (MQ=255) cAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAAcgc > 1:2026043/1‑71 (MQ=255) aCCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAAcgcg > 1:231602/1‑70 (MQ=255) ggTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTg < 1:533287/70‑1 (MQ=255) cgcgCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCAc < 1:3016105/71‑1 (MQ=255) cgcCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGa < 1:1068322/71‑1 (MQ=255) cgcCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGa < 1:1662708/71‑1 (MQ=255) cgcCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGa < 1:3199867/71‑1 (MQ=255) cgcCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGa < 1:2458236/71‑1 (MQ=255) cTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGata > 1:1247660/1‑70 (MQ=255) aTGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGatat > 1:3204433/1‑68 (MQ=255) aTGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATgg > 1:2012472/1‑70 (MQ=255) aTGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATg < 1:2610158/69‑1 (MQ=255) aTGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGa > 1:2741709/1‑71 (MQ=255) aTGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGa > 1:1927011/1‑71 (MQ=255) aTGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGa > 1:1605473/1‑71 (MQ=255) aTGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGa > 1:1538519/1‑71 (MQ=255) gggCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGt < 1:2507764/71‑1 (MQ=255) gggCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGt < 1:2542891/71‑1 (MQ=255) gggCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGt < 1:20605/71‑1 (MQ=255) gTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATtga < 1:2031371/70‑1 (MQ=255) | GCGGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATTGA > W3110S.gb/1463440‑1463568 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |