| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,521,063 | C→T | 100% | S108F (TCC→TTC) | yncB → | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,521,063 | 0 | C | T | 83.9% | 72.8 / 8.0 | 31 | S108F (TCC→TTC) | yncB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/5); new base T (7/19); total (7/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCATCCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGG > W3110S.gb/1520998‑1521132 | tcatcCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtg < 1:1250467/71‑1 (MQ=255)tcatcCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtg < 1:707732/71‑1 (MQ=255)tcatcCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtg < 1:690449/71‑1 (MQ=255) catcCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtg < 1:3144545/70‑1 (MQ=255) atcCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtggt < 1:922920/71‑1 (MQ=255) aTTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgatg < 1:209616/69‑1 (MQ=255) aTTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgatg < 1:2925027/69‑1 (MQ=255) aTTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgatg < 1:3631480/69‑1 (MQ=255) aTTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgatg < 1:1610810/69‑1 (MQ=255) aTTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgatg < 1:1396431/69‑1 (MQ=255) tGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgatg < 1:3268126/59‑1 (MQ=255) gATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCg > 1:3301860/1‑71 (MQ=255) aTGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTTAAACTTGGCGATCATCCGCAAAATCCATCGt < 1:3604392/71‑1 (MQ=255) aTGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCATAATCCATCGt < 1:3544021/71‑1 (MQ=255) aTGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGt < 1:2116942/71‑1 (MQ=255) aTGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGt < 1:3554365/71‑1 (MQ=255) aTGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGt < 1:1557720/71‑1 (MQ=255) tGGCAGGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTg > 1:3440461/1‑71 (MQ=255) tGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGa < 1:520862/40‑1 (MQ=255) tGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGa < 1:9683/40‑1 (MQ=255) tGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGa < 1:1899434/40‑1 (MQ=255) tGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTg > 1:2334029/1‑71 (MQ=255) gCAAGACTATGACATATTCAGTGGTGATGATCTGGTGaaa > 1:3177374/1‑40 (MQ=255) gCAAGACTATGACATATTCAGTGGTGATGATCTGGTGaaa > 1:1096030/1‑40 (MQ=255) aCATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGgtgt > 1:2218516/1‑70 (MQ=255) aCATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGtgtg > 1:1364129/1‑71 (MQ=255) cATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGc < 1:1579156/71‑1 (MQ=255) tCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAgg < 1:2253660/71‑1 (MQ=255) tCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAgg < 1:2240423/71‑1 (MQ=255) tCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAgg < 1:2017360/71‑1 (MQ=255) tCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAgg < 1:172071/71‑1 (MQ=255) | TCATCCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGG > W3110S.gb/1520998‑1521132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |