| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,581,839 | C→T | 100% | M222I (ATG→ATA) | ydeM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,581,839 | 0 | C | T | 97.2% | 104.2 / ‑3.9 | 36 | M222I (ATG→ATA) | ydeM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (24/10); total (25/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGAC > W3110S.gb/1581779‑1581906 | aaaCTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACtt < 1:2985774/69‑1 (MQ=255) aaCTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGcc < 1:3221589/71‑1 (MQ=255) cTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCa > 1:3700569/1‑70 (MQ=255) cGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGc < 1:3456394/71‑1 (MQ=255) aaaaTTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTgg > 1:1930790/1‑70 (MQ=255) cacaTCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaa > 1:828489/1‑70 (MQ=255) cacaTCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaa > 1:2600817/1‑70 (MQ=255) cacaTCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaa > 1:246452/1‑70 (MQ=255) cacaTCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaa > 1:1890892/1‑70 (MQ=255) cacaTCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaa > 1:1773932/1‑70 (MQ=255) caTCGTTTTTAACCCATTGCATAAAAATGGTTGAtata < 1:3203585/38‑1 (MQ=255) caTCGTTTTTAACCCATTGCATAAAAATGGTTGAtata < 1:959005/38‑1 (MQ=255) caTCGTTTTTAACCCATTGCATAAAAATGGTTGAtata < 1:1596415/38‑1 (MQ=255) caTCGTTTTTAACCCATTGCATAAAAATGGTTGAtata < 1:2601197/38‑1 (MQ=255) caTCGTTTTTAACACATTGCATAAAAATGGTTGAtata < 1:394675/38‑1 (MQ=38) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGc < 1:3005661/50‑1 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGc > 1:1639636/1‑50 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAAt > 1:369173/1‑70 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAAt > 1:3116485/1‑70 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAAt > 1:2662443/1‑70 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATc < 1:3596451/71‑1 (MQ=255) gTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCa < 1:202212/70‑1 (MQ=255) tAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGAt < 1:2326670/70‑1 (MQ=255) ccgaTTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGACACAGAAAAATCAATGATACgg < 1:1367824/68‑1 (MQ=255) ttGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTgg < 1:105505/41‑1 (MQ=255) gCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa > 1:76672/1‑60 (MQ=255) gCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa > 1:2413272/1‑60 (MQ=255) gCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa > 1:185065/1‑60 (MQ=255) gCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa > 1:1057179/1‑60 (MQ=255) cATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATgtgt < 1:1754753/71‑1 (MQ=255) aaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGca > 1:3186433/1‑40 (MQ=255) aaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt > 1:2463771/1‑63 (MQ=255) aaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt > 1:2852540/1‑68 (MQ=255) aaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt > 1:2483971/1‑68 (MQ=255) aaaaTGGTTGAAATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt > 1:105625/1‑68 (MQ=255) tGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATa > 1:1898225/1‑53 (MQ=255) gTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 1:2522762/47‑1 (MQ=255) tGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa > 1:3148540/1‑47 (MQ=255) tGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa > 1:2431237/1‑47 (MQ=255) tGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa > 1:1975496/1‑47 (MQ=255) tGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGAc > 1:267340/1‑71 (MQ=255) | AAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGAC > W3110S.gb/1581779‑1581906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |