| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,588,010 | T→G | 100% | D64A (GAC→GCC) | ydeP ← | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,588,010 | 0 | T | G | 93.7% | 154.3 / ‑1.4 | 47 | D64A (GAC→GCC) | ydeP | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base G (24/20); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGAAAGAGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTAT > W3110S.gb/1587942‑1588076 | agaaagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCg < 1:1269654/71‑1 (MQ=255)agaaagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCg < 1:2554822/71‑1 (MQ=255) aagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAAt < 1:1363185/71‑1 (MQ=255) aagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAAt < 1:3717179/71‑1 (MQ=255) gCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGc < 1:559165/71‑1 (MQ=255) gTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCAc < 1:1909709/71‑1 (MQ=255) gTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCAc < 1:2481610/71‑1 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCAc < 1:1311804/68‑1 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCAc < 1:2979571/68‑1 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgt > 1:876426/1‑71 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgt > 1:3523871/1‑71 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgt > 1:3670430/1‑71 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgt > 1:3714086/1‑71 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgt > 1:2680201/1‑71 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgt > 1:2636549/1‑71 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgt > 1:1910049/1‑71 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgt > 1:166607/1‑71 (MQ=255) cTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCt < 1:493111/71‑1 (MQ=255) cTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCt < 1:1324205/71‑1 (MQ=255) cTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgt < 1:1847655/65‑1 (MQ=255) cTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCtagg < 1:481314/71‑4 (MQ=255) aTCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCTAATGACGCACTGTGCTTAgg < 1:3092344/69‑1 (MQ=255) aTCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAgg < 1:1352079/69‑1 (MQ=255) aTCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAgg < 1:3191785/69‑1 (MQ=255) aTCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAgg < 1:1367587/69‑1 (MQ=255) aCTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCa > 1:2884946/1‑71 (MQ=255) aCTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCa > 1:382592/1‑71 (MQ=255) aCTTCCCAGGCGATTGCTTTTGCGCCGTTTTCAAAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCa > 1:1910409/1‑71 (MQ=255) cccAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGAt > 1:2406273/1‑60 (MQ=255) cccAGGCGATTACTTTTGCGCCGTTTTGACAAATGGCGAATGACGCACTGTGCTTAGGAt > 1:2406376/1‑60 (MQ=255) gATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCg < 1:3007747/71‑1 (MQ=255) tGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGa > 1:2651799/1‑70 (MQ=255) gCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGa > 1:723532/1‑69 (MQ=255) gCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGa > 1:2805847/1‑69 (MQ=255) gCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGa > 1:3372995/1‑69 (MQ=255) gCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGa > 1:1920799/1‑69 (MQ=255) gCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGa > 1:1667872/1‑69 (MQ=255) ttGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGAt > 1:3122063/1‑45 (MQ=255) ttGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGAt > 1:1012810/1‑45 (MQ=255) ttGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGAt > 1:533695/1‑45 (MQ=255) gtGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGTACAGTc < 1:1116/69‑1 (MQ=255) ttttCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCg < 1:3434145/55‑1 (MQ=255) ttttCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCg < 1:2296648/55‑1 (MQ=255) ttCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTg > 1:3651921/1‑71 (MQ=255) caAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCtt < 1:3371177/71‑1 (MQ=255) caAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCtt < 1:3007081/71‑1 (MQ=255) aTGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTAt > 1:811656/1‑70 (MQ=255) | AGAAAGAGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTAT > W3110S.gb/1587942‑1588076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |