| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,595,724 | G→A | 72.7% | pseudogene (56/1401 nt) | ydeU ← | ECK1502:JW1502:b1509; conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,595,724 | 0 | G | A | 72.7% | 85.5 / 39.3 | 55 | pseudogene (56/1401 nt) | ydeU | ECK1502:JW1502:b1509; conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (11/4); new base A (29/11); total (40/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGT > W3110S.gb/1595657‑1595788 | cATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGATCGTCATCAGGCCATTGTTTCCGGta > 1:199165/1‑70 (MQ=255)cATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAta > 1:214287/1‑70 (MQ=255)cATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAta > 1:3196365/1‑70 (MQ=255)cATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAta > 1:2998657/1‑70 (MQ=255)cATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAta < 1:3370209/70‑1 (MQ=255) aTCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGtat < 1:1324646/70‑1 (MQ=255) aTCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGtat < 1:3115459/70‑1 (MQ=255) aTCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGtat < 1:1619632/70‑1 (MQ=255) aTCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGtat < 1:2588005/70‑1 (MQ=255) aTCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAtata < 1:3377545/71‑1 (MQ=255) aTCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAtata < 1:373866/71‑1 (MQ=255) tCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAg < 1:1754927/66‑1 (MQ=255) ggTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAta > 1:854416/1‑61 (MQ=255) tGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTa < 1:542860/71‑1 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGt > 1:2380953/1‑68 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGt > 1:868828/1‑68 (MQ=255) aTCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAtata > 1:3541957/1‑51 (MQ=255) aTCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAtata > 1:3164196/1‑51 (MQ=255) aTCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAtata > 1:2115321/1‑51 (MQ=255) aTCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGAtata > 1:572785/1‑51 (MQ=255) aTCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTCAGTa > 1:1740074/1‑71 (MQ=255) tCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTCAGTa < 1:335919/70‑1 (MQ=255) tCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTCAGTa < 1:739576/70‑1 (MQ=255) ttGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTcccccc > 1:2364966/1‑71 (MQ=255) ttGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTcccccc > 1:2934021/1‑71 (MQ=255) ttGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTcccccc > 1:1711710/1‑71 (MQ=255) tcatcaGGCCATTGTTTCCGATATAGTTGCCGTTaa > 1:3651252/1‑36 (MQ=255) tcatcaGGCCATTGTTTCCGATATAGTTGCCGTTaa > 1:343723/1‑36 (MQ=255) tcatcaGGCCATTGTTTCCGATATAGTTGCCGTTaa > 1:385255/1‑36 (MQ=255) tcatcaGGCCATTGTTTCCGATATAGTTGCCGTTaa > 1:106504/1‑36 (MQ=255) tcatcaGGCCATTGTTTCCGATATAGTTGCCGTTaa > 1:2898696/1‑36 (MQ=255) tcatcaGGCCATTGTTTCCGATATAGTTGCCGTTaa > 1:1010944/1‑36 (MQ=255) tcatcaGGCCATTGTTTCCGATATAGTTGCCGTTaa > 1:1582206/1‑36 (MQ=255) caGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCgg > 1:1518382/1‑51 (MQ=255) caGCCCATTGTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCggg > 1:3319493/1‑51 (MQ=255) aGGCCATTGTTTCCGATATAGTTGCCGTTAACGGt > 1:3149855/1‑35 (MQ=255) aGGCCATTGTTTCCGATATAGTTGCCGTTAACGGt > 1:3154102/1‑35 (MQ=255) aTTGTTTCCGATATAGTTGCCGTTAACGGTCAGTACa > 1:3134503/1‑37 (MQ=255) aTTGTTTCCGATATAGTTGCCGTTAACGGTCAGTACa > 1:750684/1‑37 (MQ=255) ttGTTTCCGATATAGTTTCCGTTAACGGTCAGTACATTCCCCGGttt < 1:1934497/47‑1 (MQ=255) ttGTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCGGttt < 1:3325743/47‑1 (MQ=255) ttGTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCGGttt < 1:3001638/47‑1 (MQ=255) tGTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTccccca > 1:430757/1‑51 (MQ=255) tGTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAAc > 1:2825548/1‑70 (MQ=255) agTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCAATTGAAc > 1:3621332/2‑70 (MQ=255) gTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCg > 1:1290325/1‑41 (MQ=255) gTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTccccc > 1:3478921/1‑50 (MQ=255) gTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTccccc > 1:898931/1‑50 (MQ=255) gTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTcccc > 1:193857/1‑49 (MQ=255) gTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTcccc > 1:1513528/1‑49 (MQ=255) gTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTcccc > 1:3468545/1‑49 (MQ=255) gTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTcccc > 1:2758203/1‑49 (MQ=255) gTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCg > 1:562217/1‑41 (MQ=255) gTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTAt > 1:269768/1‑59 (MQ=255) tttCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGt < 1:1736706/71‑1 (MQ=255) | CATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGT > W3110S.gb/1595657‑1595788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |