Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,620,710 | C→T | 87.3% | intergenic (‑88/‑124) | marC ← / → marR | predicted transporter/DNA‑binding transcriptional repressor |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,620,710 | 0 | C | T | 87.3% | 145.5 / 13.0 | 55 | intergenic (‑88/‑124) | marC/marR | predicted transporter/DNA‑binding transcriptional repressor |
Reads supporting (aligned to +/- strand): ref base C (2/5); new base T (35/13); total (37/18) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.17e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 |
TATCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACT > W3110S.gb/1620642‑1620776 | tATCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTAt > 1:2809321/1‑71 (MQ=255) tATCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTAt > 1:1860592/1‑71 (MQ=255) tATCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTAt > 1:1398310/1‑71 (MQ=255) tATCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTAt > 1:873481/1‑71 (MQ=255) tATCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCAt > 1:111018/1‑71 (MQ=255) tCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATct > 1:1841489/1‑70 (MQ=255) tCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCg > 1:2687191/1‑71 (MQ=255) tCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCg > 1:927426/1‑71 (MQ=255) ttATCAGCGAAAAGTATAAGGGGTAAACAATGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg < 1:2304413/70‑1 (MQ=255) ttATCAGCGAAAAGTATAAGGGGTAAAAAATGATAAAGTGTCACTCTTTAGCTAGCCTTGTATc > 1:1064207/1‑64 (MQ=255) tataAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAAcc < 1:609724/71‑1 (MQ=255) tataAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAAcc < 1:1411893/71‑1 (MQ=255) tataAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAAcc < 1:2047310/71‑1 (MQ=255) aGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAACCGAtt > 1:2162513/1‑71 (MQ=255) gggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTg < 1:125918/61‑1 (MQ=255) tAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt < 1:2980475/66‑1 (MQ=255) tAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt < 1:2609094/66‑1 (MQ=255) tAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt < 1:3436617/66‑1 (MQ=255) tAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt < 1:788686/66‑1 (MQ=255) tAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt < 1:1541791/66‑1 (MQ=255) aaCAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACaa < 1:2227196/50‑1 (MQ=255) aaCAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACaa < 1:3332574/50‑1 (MQ=255) tgtCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGt > 1:2001613/1‑71 (MQ=255) tgtCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGt > 1:969064/1‑71 (MQ=255) tgtCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGt > 1:1128618/1‑71 (MQ=255) gtCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGt > 1:3249205/1‑70 (MQ=255) gtCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGt > 1:1526698/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc < 1:2894340/70‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAACAAAAc > 1:3514254/1‑36 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAat > 1:397491/1‑71 (MQ=255) aCTCTTTAGCTAGCCTTGTATAGCATTGAACAAAAc > 1:1074532/1‑36 (MQ=38) tctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAatt > 1:813872/1‑70 (MQ=255) tctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAatt > 1:1549193/1‑70 (MQ=255) tctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAatt > 1:1952347/1‑70 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:857210/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:834587/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:2016086/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:367010/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:54897/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattc > 1:3357126/1‑70 (MQ=255) tttAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt > 1:3148608/1‑43 (MQ=255) tttAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt > 1:9502/1‑43 (MQ=255) tttAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt > 1:606875/1‑43 (MQ=255) tttAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt > 1:2458060/1‑43 (MQ=255) tttAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGAtt > 1:320381/1‑43 (MQ=255) tagctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattcatt > 1:2749698/1‑70 (MQ=255) tagctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattcatt > 1:1467577/1‑70 (MQ=255) agctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattcattc < 1:3070376/70‑1 (MQ=255) gctagcCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAAttcattca < 1:1306009/70‑1 (MQ=255) cTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTgg > 1:1869965/1‑46 (MQ=255) cTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTgg > 1:1747536/1‑46 (MQ=255) cTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTgg > 1:865037/1‑46 (MQ=255) cTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACt < 1:3667635/71‑1 (MQ=255) cTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACt < 1:3211833/71‑1 (MQ=255) cTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACt < 1:1287370/71‑1 (MQ=255) | TATCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACT > W3110S.gb/1620642‑1620776 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |