| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,657,562 | T→C | 100% | I14T (ATT→ACT) | ynfB → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,657,562 | 0 | T | C | 84.2% | 94.7 / 10.3 | 38 | I14T (ATT→ACT) | ynfB | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base C (8/24); total (9/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAATCATTCATACAGGGAATGAATTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTATTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGTTCATGCCGAAACTAACAAACTGGTGATT > W3110S.gb/1657497‑1657626 | tAATCATTCATACAGGGAATGAATTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTActct < 1:976394/69‑1 (MQ=255)tAATCATTCATACAGGGAATGAATTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTActct < 1:3323111/69‑1 (MQ=255)tAATCATTCATACAGGGAATGAATTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTActct < 1:3319826/69‑1 (MQ=255)tAATCATTCATACAGGGAATGAATTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTctg < 1:1190392/70‑1 (MQ=255) attcatACAGGGAATGAATTATGAAAATCACTCTCAGCAAACGAATCTGCCTGCTCGCTACTCTGCTGCCt > 1:1903985/1‑71 (MQ=255) attcatACAGGGAATGAATTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCt > 1:735684/1‑71 (MQ=255) gggAATTAATTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTgcg < 1:228190/66‑1 (MQ=255) tgaatTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCAtt > 1:2892875/1‑71 (MQ=255) tgaatTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCAtt > 1:2058065/1‑71 (MQ=255) tgaatTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCAt > 1:2801943/1‑70 (MQ=255) aatTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTATTCtgctgc > 1:2655673/1‑53 (MQ=255) tGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGcac < 1:3310276/70‑1 (MQ=255) tCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:1700351/71‑1 (MQ=255) tCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:1817852/71‑1 (MQ=255) tCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:1963669/71‑1 (MQ=255) tCACTCTCAGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:2088759/71‑1 (MQ=255) aGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTg < 1:3651712/61‑1 (MQ=255) aGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTg < 1:1980061/61‑1 (MQ=255) aGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTg < 1:1809996/61‑1 (MQ=255) aGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTg < 1:1725918/61‑1 (MQ=255) aGCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGTTCATGCCGa < 1:214374/71‑1 (MQ=255) gCAAACGAATCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTg < 1:2295814/60‑1 (MQ=255) aaTCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGTTCATGCCGAAACTAACa < 1:2991261/71‑1 (MQ=255) aaTCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGTTCATGCCGAAACTAACa < 1:770190/71‑1 (MQ=255) aaTCGGCCTGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGTTCATGCCGAAACTAACa < 1:787726/71‑1 (MQ=255) tGCTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGTTCATGCCGAAACTAACAAACTGGt > 1:253814/1‑70 (MQ=255) cTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:3681573/45‑1 (MQ=255) cTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:604216/45‑1 (MQ=255) cTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:1530454/45‑1 (MQ=255) cTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:115555/45‑1 (MQ=255) cTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:2503066/45‑1 (MQ=255) cTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:2215156/45‑1 (MQ=255) cTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:1487847/45‑1 (MQ=255) cTCGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGTTCATGCCGAAACTAACAAACTGGTGAt > 1:1262668/1‑71 (MQ=255) cGCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAAATGTTCATGCCGAAACTAACAAACTGGTGAtt > 1:2528975/1‑70 (MQ=255) gCTACTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGTTCATGCCGAAACTAACAAACTGGTGAtt < 1:2892397/69‑1 (MQ=255) tATTCTGCTGCCTTGCGCACTGGCATTGAGCACATCTGtt < 1:2274417/40‑1 (MQ=255) tATTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:1431751/40‑1 (MQ=255) tATTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:2485703/40‑1 (MQ=255) tATTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:217521/40‑1 (MQ=255) tATTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGtt < 1:2062981/40‑1 (MQ=255) | TAATCATTCATACAGGGAATGAATTATGAAAATCACTCTCAGCAAACGAATCGGCCTGCTCGCTATTCTGCTGCCTTGCGCACTGGCATTGAGCACAACTGTTCATGCCGAAACTAACAAACTGGTGATT > W3110S.gb/1657497‑1657626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |