| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,692,839 | G→A | 67.9% | G425D (GGT→GAT) | ydgA → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,692,839 | 0 | G | A | 67.9% | 62.8 / 46.9 | 53 | G425D (GGT→GAT) | ydgA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (8/9); new base A (23/13); total (31/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
GACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCA > W3110S.gb/1692783‑1692905 | gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGAt > 1:2121348/1‑70 (MQ=255)gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGAt > 1:1581095/1‑70 (MQ=255) aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCa < 1:1693948/71‑1 (MQ=255) ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCg < 1:1649815/71‑1 (MQ=255) tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCg > 1:2429299/1‑70 (MQ=255) tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCg > 1:395213/1‑70 (MQ=255) tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCg > 1:198413/1‑70 (MQ=255) tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCg > 1:2909866/1‑70 (MQ=255) tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCg > 1:151228/1‑70 (MQ=255) tGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGaaaaaa > 1:2585166/1‑71 (MQ=255) gATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACt > 1:2530968/1‑71 (MQ=255) gATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACt > 1:251217/1‑71 (MQ=255) gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTAt > 1:3612037/1‑42 (MQ=255) gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTAt > 1:596484/1‑42 (MQ=255) gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTAt > 1:2543725/1‑42 (MQ=255) gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTAt > 1:94159/1‑42 (MQ=255) aCTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGa < 1:3178434/47‑1 (MQ=255) aCTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGa < 1:3246399/47‑1 (MQ=255) aCTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGa < 1:947243/47‑1 (MQ=255) aTGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTg < 1:312786/70‑1 (MQ=255) aCTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGa < 1:2657434/55‑1 (MQ=255) gTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTg < 1:2353675/67‑1 (MQ=255) gTAGCGAAGCTGGAAGGTTATCAGGAAGATC‑AGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTg < 1:3233855/66‑1 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCa > 1:3695919/1‑68 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCAt > 1:387296/1‑69 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCAt > 1:3079708/1‑69 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCAt > 1:1551785/1‑69 (MQ=255) tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCAt > 1:2211095/1‑69 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCa > 1:1565284/1‑67 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCa > 1:448289/1‑67 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCa > 1:1867227/1‑67 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCa > 1:676910/1‑67 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCa > 1:1273133/1‑67 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAg > 1:3693156/1‑71 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAg > 1:3689125/1‑71 (MQ=255) aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAg > 1:3292297/1‑71 (MQ=255) aGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAg > 1:104030/1‑71 (MQ=255) aGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAg > 1:3160562/1‑71 (MQ=255) aGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAg > 1:2494339/1‑71 (MQ=255) aGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAg > 1:218677/1‑71 (MQ=255) gCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTg < 1:1838447/64‑1 (MQ=255) gCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTg < 1:731210/64‑1 (MQ=255) gCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTg < 1:903296/64‑1 (MQ=255) gCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTg < 1:961587/64‑1 (MQ=255) gCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGc < 1:315772/71‑1 (MQ=255) gCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGc < 1:1864433/71‑1 (MQ=255) cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa < 1:3200283/71‑1 (MQ=255) cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa < 1:840553/71‑1 (MQ=255) cGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCa < 1:1030755/71‑1 (MQ=255) gAAGGTTATCAGGAAGATCAAGCTAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCa < 1:127434/71‑1 (MQ=255) gAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCa < 1:154255/71‑1 (MQ=255) aaGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCa < 1:3088100/70‑1 (MQ=255) aaGATTATCAGGAAGATCAAGCGAAAAAACTGGCGa < 1:2530422/36‑1 (MQ=255) | GACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCA > W3110S.gb/1692783‑1692905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |