| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,761,776 | C→T | 100% | L154L (CTG→CTA) | sufS ← | selenocysteine lyase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,761,776 | 0 | C | T | 87.0% | 119.3 / 10.2 | 46 | L154L (CTG→CTA) | sufS | selenocysteine lyase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (1/5); new base T (30/10); total (31/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCACGTTGGAGACATGAGTAATTGCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATC > W3110S.gb/1761708‑1761845 | aGCACGTTGGAGACATGAGTAATTGCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAg < 1:899447/71‑1 (MQ=255) cGTTGGAGACATGAGTAATTGCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGc < 1:2292353/71‑1 (MQ=255) aGTAATTGCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCgg > 1:517990/1‑71 (MQ=255) ttGCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCTTCGGGATTg < 1:2185094/71‑1 (MQ=255) ttGCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACAATCGGGATTg < 1:2399499/71‑1 (MQ=255) gCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGa > 1:1260018/1‑70 (MQ=255) gCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGa > 1:2192032/1‑70 (MQ=255) gCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGa > 1:423555/1‑70 (MQ=255) gCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAg > 1:1689097/1‑71 (MQ=255) gCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAg > 1:303571/1‑71 (MQ=255) gCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAg > 1:2135671/1‑71 (MQ=255) aGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATcaca > 1:2972882/1‑70 (MQ=255) gTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACg > 1:1291042/1‑71 (MQ=255) gTTTTCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACg > 1:646546/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTAc > 1:3040917/1‑43 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTAc > 1:799913/1‑43 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:2806427/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:390433/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:910195/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:3045200/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:3059165/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:3060315/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:2183279/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:1165914/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:1706436/1‑71 (MQ=255) ttCTCATCAAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAg > 1:1899452/1‑71 (MQ=255) atcaAACAGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATAGGGATTGAGCGGGATCACACGCAGctc < 1:2312383/69‑1 (MQ=255) aGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCaa > 1:1913599/1‑70 (MQ=255) aGCGTAGGCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAAc > 1:1864037/1‑71 (MQ=255) ggcggcGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTg > 1:3373779/1‑70 (MQ=255) ggCAGCGTCTCTAGTTGCAACGTAGCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTgc > 1:3267053/1‑71 (MQ=255) ggCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTgc > 1:2800298/1‑71 (MQ=255) ggCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTgc > 1:1922538/1‑71 (MQ=255) ggCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTgc > 1:2599840/1‑71 (MQ=255) ggCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTg > 1:2823891/1‑70 (MQ=255) ggCAGCGTCTCTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTg > 1:277389/1‑70 (MQ=255) cAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTgcgc < 1:2829086/71‑1 (MQ=255) cAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTgcgc < 1:122874/71‑1 (MQ=255) tctcTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCa < 1:1123141/67‑1 (MQ=255) tctcTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCa < 1:2619132/67‑1 (MQ=255) tctcTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCa < 1:2103142/67‑1 (MQ=255) tctcTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGc < 1:2281515/71‑1 (MQ=255) tctcTAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGc < 1:916294/71‑1 (MQ=255) tctcCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGc < 1:2641687/71‑1 (MQ=255) tctcCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGc < 1:2637267/71‑1 (MQ=255) tctcCAGTTCCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGc < 1:1781565/71‑1 (MQ=255) cAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATc < 1:1023567/70‑1 (MQ=255) | AGCACGTTGGAGACATGAGTAATTGCCAGCAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATC > W3110S.gb/1761708‑1761845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |