| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,775,080 | T→C | 62.9% | F285F (TTT→TTC) | ydiN → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,775,080 | 0 | T | C | 62.9% | 54.5 / 63.8 | 62 | F285F (TTT→TTC) | ydiN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (5/18); new base C (17/22); total (22/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01 | |||||||||||
TGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAA > W3110S.gb/1775020‑1775145 | tgtCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTg < 1:2628881/68‑1 (MQ=255)tgtCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTg < 1:316845/68‑1 (MQ=255) ttagCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGcaacaa < 1:575571/67‑1 (MQ=255) ttGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAAt < 1:315190/70‑1 (MQ=255) ttGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAAt < 1:2453133/70‑1 (MQ=255) gCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATaa < 1:2891280/70‑1 (MQ=255) gCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATaa < 1:545584/70‑1 (MQ=255) ctactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGcaacaa < 1:1019559/61‑1 (MQ=255) ctactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGcaacaa < 1:2946720/61‑1 (MQ=255) ctactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGcaacaa < 1:2875472/61‑1 (MQ=255) ctactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGcaacaa < 1:2528973/61‑1 (MQ=255) ctactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGcaacaa < 1:1077711/61‑1 (MQ=255) ctactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACagcagc < 1:2829033/71‑1 (MQ=255) ctactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACagcagc > 1:73693/1‑71 (MQ=255) ctactGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACagcagc < 1:3359442/71‑1 (MQ=255) actGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATa > 1:1498527/1‑61 (MQ=255) actGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCaa > 1:2216894/1‑42 (MQ=255) actGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATaa > 1:288804/1‑62 (MQ=255) ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAt > 1:3675817/1‑71 (MQ=255) aaaaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAActc > 1:794664/1‑41 (MQ=255) aaaaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAActc > 1:405310/1‑41 (MQ=255) aaaaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:2027431/69‑1 (MQ=255) aaaaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:1650988/69‑1 (MQ=255) aaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTTGCAACAATAACAGCAGCCATTATCt < 1:1629176/71‑1 (MQ=255) aaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCt < 1:6467/71‑1 (MQ=255) aaaaaTGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCt < 1:3201797/71‑1 (MQ=255) ggTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCt < 1:2577405/65‑1 (MQ=255) tCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:2153422/50‑1 (MQ=255) tCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:2149247/50‑1 (MQ=255) tCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:1721801/50‑1 (MQ=255) tCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:2850391/50‑1 (MQ=255) tCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:3700146/50‑1 (MQ=255) tCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:2815976/50‑1 (MQ=255) tCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:3537718/50‑1 (MQ=255) tCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAtt < 1:334523/50‑1 (MQ=255) tGGGCTAATGTATTCAACTCTGCACTGGCAACAATaa > 1:1521317/1‑37 (MQ=255) tGGGCTAATGTATTCAACTCTGCACTGGCAACAATa > 1:1464864/1‑36 (MQ=255) tGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACt > 1:2992422/1‑71 (MQ=255) tGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACt > 1:2287028/1‑71 (MQ=255) tGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACt > 1:214261/1‑71 (MQ=255) agggCTAATGTATTCAACTCTGCACTGGCAACAATaa > 1:1197468/2‑37 (MQ=38) ggCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTgg < 1:2805637/71‑1 (MQ=255) aaTGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTa > 1:115113/1‑43 (MQ=255) aaTGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATc > 1:2799939/1‑45 (MQ=255) aaTGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATc > 1:1080418/1‑45 (MQ=255) aaTGTACTCAACTCTGCACTGGCAACAATAACAGCCGCCAtt < 1:3055688/42‑1 (MQ=37) aTGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGc < 1:2284257/71‑1 (MQ=255) tGTATTTAACTCTGCACTGGCAACAATAACagcagc < 1:2734866/36‑1 (MQ=255) tGTATTTAACTCTGCACTGGCAACAATAACagcagc < 1:1429207/36‑1 (MQ=255) tGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc < 1:1051884/37‑1 (MQ=255) tGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc < 1:2521815/37‑1 (MQ=255) tGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc < 1:1484565/37‑1 (MQ=255) tGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc < 1:1422639/37‑1 (MQ=255) tGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCt < 1:3133355/44‑1 (MQ=255) tGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCt < 1:822292/44‑1 (MQ=255) gTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCaa > 1:2830153/1‑71 (MQ=255) gTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCaa > 1:468684/1‑71 (MQ=255) tATTTAACTCTGCACTGGCAATAATAACAGCAGCCa > 1:1297513/1‑36 (MQ=255) tATTTAACTCTGCACTGGCAACAATAACAGCAGcc > 1:903345/1‑35 (MQ=255) tATTTAACTCTGCACTGGCAACAATAACAGCAGCCa > 1:1328206/1‑36 (MQ=255) tATTTAACTCTGCACTGGCAACAATAACAGCAGCCa > 1:1075759/1‑36 (MQ=255) tATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTtc < 1:842210/55‑1 (MQ=255) | TGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAA > W3110S.gb/1775020‑1775145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |