| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,887,874 | T→C | 100% | F106L (TTT→CTT) | yeaX → | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,887,874 | 0 | T | C | 86.3% | 140.3 / 11.4 | 51 | F106L (TTT→CTT) | yeaX | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (7/0); new base C (31/13); total (38/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCT > W3110S.gb/1887808‑1887936 | tCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTgc > 1:3015153/1‑71 (MQ=255) cGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:1783868/71‑1 (MQ=255) ttttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAtt > 1:122848/1‑71 (MQ=255) agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc > 1:2678780/1‑70 (MQ=255) ggTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:104994/54‑1 (MQ=255) ggTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:2100294/54‑1 (MQ=255) ggTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:420829/54‑1 (MQ=255) tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAg > 1:1750350/1‑71 (MQ=255) tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAg > 1:3179295/1‑71 (MQ=255) tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAg > 1:1062321/1‑71 (MQ=255) tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAg > 1:2382214/1‑71 (MQ=255) aaaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTaa > 1:1443539/1‑53 (MQ=255) aaaaGTGGGCGATCGGTTAACGATTTCAAAGCCTAATAACCTGCTTGCGCTaa > 1:2691187/1‑53 (MQ=39) aaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCa > 1:3229783/1‑60 (MQ=255) aaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCa > 1:3109880/1‑71 (MQ=255) cGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTa > 1:3241105/1‑71 (MQ=255) cGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTa > 1:1309662/1‑71 (MQ=255) cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTaa > 1:1319085/1‑40 (MQ=255) cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTaa > 1:2153165/1‑40 (MQ=255) cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:629015/1‑71 (MQ=255) cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:3010822/1‑71 (MQ=255) ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgcg < 1:2358358/71‑1 (MQ=255) aaCGATTTCAACGCCTAATAACCTGCTTGCGCTaa > 1:3554563/1‑35 (MQ=255) aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:951999/1‑71 (MQ=255) aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:1513899/1‑71 (MQ=255) aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:3672399/1‑71 (MQ=255) aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:3550059/1‑71 (MQ=255) aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:3276910/1‑71 (MQ=255) aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:2256406/1‑71 (MQ=255) aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:2994346/1‑71 (MQ=255) aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcgg < 1:784903/71‑1 (MQ=255) aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcgg < 1:1029054/71‑1 (MQ=255) aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcgg < 1:3644861/71‑1 (MQ=255) aCGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt < 1:2143488/71‑1 (MQ=255) aCGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt < 1:3306114/71‑1 (MQ=255) ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcg > 1:79083/1‑48 (MQ=255) ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCt > 1:3655807/1‑71 (MQ=255) ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCt > 1:920369/1‑71 (MQ=255) ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCt > 1:2840976/1‑71 (MQ=255) ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCt > 1:3051749/1‑71 (MQ=255) ataaCCTGTTTGCGCTAATTCCATCAGCCAGAAAGCATc > 1:3364703/1‑39 (MQ=255) ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:2356240/1‑49 (MQ=255) ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:818088/1‑49 (MQ=255) ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:898621/1‑49 (MQ=255) ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:1693093/1‑49 (MQ=255) ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:2554326/1‑49 (MQ=255) ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcg > 1:1469608/1‑48 (MQ=255) ataaCCTGCTTGCGCTAATTCCCTAAGCCAGAAAGCATCTGTTTATcgc > 1:3653869/1‑49 (MQ=255) taaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTgg < 1:2298909/60‑1 (MQ=255) taaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTgg < 1:3526463/60‑1 (MQ=255) taaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTgg < 1:515787/60‑1 (MQ=255) | TCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCT > W3110S.gb/1887808‑1887936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |