| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,927,287 | T→C | 100% | L45P (CTT→CCT) | yobB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,927,287 | 0 | T | C | 87.5% | 64.0 / 1.2 | 24 | L45P (CTT→CCT) | yobB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (8/13); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGT > W3110S.gb/1927224‑1927349 | atcatcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttctt < 1:2121064/69‑1 (MQ=255) gTTTGTGAGGGCCGCCGCCAGGCGACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt < 1:3495237/70‑1 (MQ=255) tttGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGcctt < 1:1135058/55‑1 (MQ=255) tttGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGcctt < 1:3298881/55‑1 (MQ=255) tttGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGcctt < 1:591030/55‑1 (MQ=255) tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGat < 1:1635841/70‑1 (MQ=255) gtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGat < 1:1203792/69‑1 (MQ=255) tgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGatta < 1:1219626/70‑1 (MQ=255) tgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGatta < 1:612698/70‑1 (MQ=255) ggCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCtt < 1:3104580/71‑1 (MQ=255) ggCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCtt < 1:3570594/71‑1 (MQ=255) gCAACAATGTCAGCTGTTAGTTTTTCCATCGctttctt < 1:3372274/38‑1 (MQ=255) gCAACAATGTCAGCTGTTAGTTTTTCCATCGctttctt < 1:3319595/38‑1 (MQ=255) gCAACAATGTCAGCTGTTAGTTTTTCCATCGctttctt < 1:2575899/38‑1 (MQ=255) gCAACAATGTCAGCTGTTAGTTTTTCCATCGctttctt < 1:2571495/38‑1 (MQ=255) gCAACAATGTCAGCTGTTAGTTTTTCCATCGctttctt < 1:900648/38‑1 (MQ=255) gCAACAATGTCAGCTGTTAGTTTTTCCATCGctttctt < 1:97377/38‑1 (MQ=255) aacaaTGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCt > 1:3362366/1‑71 (MQ=255) aaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc > 1:570021/1‑67 (MQ=255) aaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc > 1:595566/1‑67 (MQ=255) aaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGcc > 1:2593090/1‑71 (MQ=255) cTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc < 1:249299/46‑1 (MQ=255) ttAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc < 1:1192645/43‑1 (MQ=255) ttAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc < 1:2702015/43‑1 (MQ=255) ttAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc < 1:1917852/43‑1 (MQ=255) ttAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTc < 1:460816/55‑1 (MQ=255) tAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAtctc > 1:1775006/1‑71 (MQ=255) aGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCACACCCGAtctct > 1:1515168/1‑71 (MQ=255) tttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCAc > 1:3095877/1‑70 (MQ=255) tCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGt > 1:3698364/1‑71 (MQ=255) tCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGt > 1:805038/1‑71 (MQ=255) ccATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGt < 1:2751007/70‑1 (MQ=255) ccATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGt < 1:1422004/70‑1 (MQ=255) cGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCt < 1:1889786/40‑1 (MQ=255) | ATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGT > W3110S.gb/1927224‑1927349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |