| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,965,064 | G→A | 100% | I567I (ATC→ATT) | flhA ← | predicted flagellar export pore protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,965,064 | 0 | G | A | 88.2% | 88.4 / 5.0 | 34 | I567I (ATC→ATT) | flhA | predicted flagellar export pore protein |
| Reads supporting (aligned to +/- strand): ref base G (0/4); new base A (18/12); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCA > W3110S.gb/1964997‑1965127 | aCTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAAt > 1:1277181/1‑70 (MQ=255)aCTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAAt > 1:1402967/1‑70 (MQ=255)aCTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAAt > 1:445676/1‑70 (MQ=255) ctgGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGgcg < 1:3071264/71‑1 (MQ=255) gggTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCa > 1:1897039/1‑71 (MQ=255) aTCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTc > 1:629924/1‑71 (MQ=255) aTCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTc > 1:2102935/1‑71 (MQ=255) tCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTcc < 1:764882/71‑1 (MQ=255) tCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTcc < 1:1818594/71‑1 (MQ=255) tCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTcc < 1:3337125/71‑1 (MQ=255) ccAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGtt > 1:856012/1‑71 (MQ=255) ccAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGtt > 1:2961131/1‑71 (MQ=255) ccAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGtt > 1:2241232/1‑71 (MQ=255) aaCGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTc > 1:2307447/1‑71 (MQ=255) aaCGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTc > 1:1482625/1‑71 (MQ=255) aaCGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTc > 1:59930/1‑71 (MQ=255) aaCGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTc > 1:1192800/1‑71 (MQ=255) gCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCgag < 1:2352381/71‑1 (MQ=255) gCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCgag < 1:2098097/71‑1 (MQ=255) gCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTc > 1:1814681/1‑68 (MQ=255) gCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCgag < 1:2629704/71‑1 (MQ=255) gaCGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGaa < 1:745355/70‑1 (MQ=255) cACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGaa < 1:26980/71‑1 (MQ=255) cACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGaa < 1:72860/71‑1 (MQ=255) cACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGaa < 1:627010/71‑1 (MQ=255) cgcACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGttt > 1:3408901/1‑61 (MQ=255) cgcACGACGGCGGTTAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGttt > 1:2078794/1‑61 (MQ=255) gTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCatat < 1:1450685/67‑1 (MQ=255) gTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATcgcg < 1:1222826/71‑1 (MQ=255) ttAATTCATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTgcg < 1:3506630/61‑1 (MQ=255) cATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCgg > 1:3442114/1‑70 (MQ=255) cATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCgg < 1:2744486/70‑1 (MQ=255) cATGTGGATCGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCgg < 1:2740067/70‑1 (MQ=255) cGCTTTGAATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCa > 1:327671/1‑71 (MQ=255) | ACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCA > W3110S.gb/1964997‑1965127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |