| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,995,272 | T→C | 100% | S524S (TCA→TCG) | uvrC ← | excinuclease UvrABC, endonuclease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,995,272 | 0 | T | C | 75.0% | 48.1 / 14.4 | 28 | S524S (TCA→TCG) | uvrC | excinuclease UvrABC, endonuclease subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base C (13/8); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.68e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACG > W3110S.gb/1995206‑1995340 | aCGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGaa < 1:902002/70‑1 (MQ=255) tCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTa < 1:1759744/71‑1 (MQ=255) cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTa < 1:799479/70‑1 (MQ=255) cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTa > 1:1452617/1‑70 (MQ=255) cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTa < 1:3457019/70‑1 (MQ=255) cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTa > 1:3058285/1‑70 (MQ=255) cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTa > 1:2140241/1‑70 (MQ=255) gcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTaaa > 1:3354826/1‑71 (MQ=255) tgtgATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTc < 1:1113308/68‑1 (MQ=255) tgtgATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTc < 1:2008714/68‑1 (MQ=255) cATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGc > 1:720475/1‑71 (MQ=255) cATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGc > 1:3113725/1‑71 (MQ=255) cATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTACGGc > 1:209855/1‑71 (MQ=255) gcgAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAAt > 1:3643174/1‑52 (MQ=255) tGCTGGATAACATGCAGCGCGAGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGaa > 1:2086581/1‑68 (MQ=255) aTAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc < 1:2546892/71‑1 (MQ=255) tAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc < 1:1791058/70‑1 (MQ=255) cATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagt > 1:3441261/1‑71 (MQ=255) cATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagt > 1:431074/1‑71 (MQ=255) cATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagt > 1:52512/1‑71 (MQ=255) tGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:1114270/1‑70 (MQ=255) cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtcca > 1:2571960/1‑70 (MQ=255) ggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACg < 1:2882099/71‑1 (MQ=255) ggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACg < 1:2724243/71‑1 (MQ=255) ggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACg < 1:1613805/71‑1 (MQ=255) ggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACg < 1:2167337/71‑1 (MQ=255) ggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACg < 1:1084714/71‑1 (MQ=255) gTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACg < 1:3665614/70‑1 (MQ=255) | ACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACG > W3110S.gb/1995206‑1995340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |