| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,998,457 | T→C | 6.5% | E171E (GAA→GAG) | sdiA ← | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,998,457 | 0 | T | C | 6.5% | 171.3 / 3.7 | 61 | E171E (GAA→GAG) | sdiA | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base T (27/30); new base C (2/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
CCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTT > W3110S.gb/1998393‑1998524 | ccgccgTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTtcatc < 1:293552/70‑1 (MQ=255) cgTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTa < 1:1088096/71‑1 (MQ=255) cgTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTa < 1:2520002/71‑1 (MQ=255) cACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACg > 1:2998948/1‑71 (MQ=255) cACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACg > 1:3106725/1‑71 (MQ=255) ccTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTa < 1:84951/65‑1 (MQ=255) cAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCAt > 1:1037191/1‑69 (MQ=255) cAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCAt > 1:1764422/1‑69 (MQ=255) aaTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTa < 1:320204/59‑1 (MQ=255) aaTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTa < 1:1302146/59‑1 (MQ=255) aaTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTa < 1:565812/59‑1 (MQ=255) aaTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTa < 1:1089327/59‑1 (MQ=255) aaTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCagag < 1:2299657/71‑1 (MQ=255) tttCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGcc < 1:2149823/71‑1 (MQ=255) tttCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGc > 1:3350406/1‑70 (MQ=255) tCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCa > 1:3163839/1‑64 (MQ=255) ttCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGAc > 1:1100355/1‑71 (MQ=255) ttCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGAc > 1:2572961/1‑71 (MQ=255) ggcgcTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACt < 1:3317878/69‑1 (MQ=255) ggcgcTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACt < 1:3313340/69‑1 (MQ=255) cgcgCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACtt > 1:3401194/1‑71 (MQ=255) cgcgCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACtt > 1:91137/1‑71 (MQ=255) cgcgCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACtt > 1:2557784/1‑71 (MQ=255) cgcgCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACtt > 1:2251742/1‑71 (MQ=255) cgcgCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACt < 1:943621/70‑1 (MQ=255) cgcgCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACt < 1:2992885/70‑1 (MQ=255) cgcgCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACt < 1:1300958/70‑1 (MQ=255) cgcgCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCACTTAAACGCATCAGAGCCATCAGACt < 1:2866675/70‑1 (MQ=255) gCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgcg < 1:1363061/69‑1 (MQ=255) tctGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAt > 1:3580911/1‑71 (MQ=255) gCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAttttt < 1:1145921/71‑1 (MQ=255) gCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAttttt < 1:805550/71‑1 (MQ=255) gCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAttttt < 1:1553249/71‑1 (MQ=255) tcatcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGt < 1:214704/56‑1 (MQ=255) tcatcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGt < 1:3336046/56‑1 (MQ=255) tcatcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGt < 1:2805331/56‑1 (MQ=255) tcatcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAttttt < 1:2869550/68‑1 (MQ=255) tcatcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAttttt < 1:1132905/68‑1 (MQ=255) tcatcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAttttt < 1:942681/68‑1 (MQ=255) tcatcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAttttt < 1:61293/68‑1 (MQ=255) catcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGt < 1:182932/55‑1 (MQ=255) atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGAc > 1:3303163/1‑40 (MQ=255) atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTaa > 1:3141385/1‑56 (MQ=255) atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTaa > 1:2213177/1‑56 (MQ=255) atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTaa > 1:1936355/1‑56 (MQ=255) atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTaa > 1:57559/1‑56 (MQ=255) atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTaa > 1:1406174/1‑56 (MQ=255) atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTaa > 1:1191808/1‑56 (MQ=255) atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTg > 1:1938933/1‑71 (MQ=255) tcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGc < 1:385932/71‑1 (MQ=255) tcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGc < 1:2543297/71‑1 (MQ=255) tcaCTATCTCATCATTTAAACGCATCAGAGCCATCAg < 1:1259545/37‑1 (MQ=255) tcaCTATCTCATCATTTAAACGCATCAGAGCCATCAg < 1:2549889/37‑1 (MQ=255) aCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAg > 1:2108631/1‑71 (MQ=255) aCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAg > 1:2104386/1‑71 (MQ=255) aCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAg > 1:3141961/1‑71 (MQ=255) aCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAg > 1:197865/1‑71 (MQ=255) aCTATCTCATCATTTAAACGCATCAGAGCCATCAGAc > 1:32270/1‑37 (MQ=255) aCTATCTCATCATTTAAACGCATCAGAGCCATCAGAc > 1:1699545/1‑37 (MQ=255) aTTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGtt > 1:2115919/1‑70 (MQ=255) aTTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGtt > 1:1166180/1‑70 (MQ=255) tttCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGtt < 1:211382/69‑1 (MQ=255) tttCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGtt < 1:550519/69‑1 (MQ=255) | CCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTT > W3110S.gb/1998393‑1998524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |