| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,999,934 | A→G | 47.4% | V6V (GTT→GTC) | yecC ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,999,934 | 0 | A | G | 47.4% | ‑3.7 / 23.1 | 19 | V6V (GTT→GTC) | yecC | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (3/7); new base G (6/3); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCT > W3110S.gb/1999870‑1999997 | gCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTc < 1:2353294/70‑1 (MQ=255) gATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTgg < 1:559692/71‑1 (MQ=255) aCCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGTtc > 1:3546335/1‑71 (MQ=255) ccGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTtct < 1:345099/71‑1 (MQ=255) gcagcaCCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTtct < 1:2848638/67‑1 (MQ=255) cagcaCCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGTtctctctc > 1:1881792/1‑71 (MQ=255) gcaCCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCAtt < 1:980261/56‑1 (MQ=255) gcaCCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCAtt < 1:3678273/56‑1 (MQ=255) aCCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGTtc > 1:3504702/1‑61 (MQ=255) aCCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTcc > 1:2421883/1‑68 (MQ=255) aCCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTgg > 1:3471846/1‑71 (MQ=255) aCCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCATTTTGGTTCTCTCTCCTgg > 1:2636486/1‑71 (MQ=255) tCTGACCGTGGAATTTTTTCACCAGGTTCTTGACTTCAATGGCACTCa < 1:734929/48‑1 (MQ=255) ttttttCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATtct > 1:3061056/1‑70 (MQ=255) ttttttCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATtct > 1:2020741/1‑70 (MQ=255) aGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTc > 1:2681706/1‑71 (MQ=255) ggTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCt < 1:712275/71‑1 (MQ=255) ttCTTAACTTCAATGGCACTCATTTTGGTtctctc < 1:760993/35‑1 (MQ=255) ttCTTAACTTCAATGGCACTCATTTTGGTtctctc < 1:816635/35‑1 (MQ=255) | GCTTTACCTCAAGGTCGATACCGTGCAGCACCGTCTGACCGTGGAATTTTTTCACCAGGTTCTTAACTTCAATGGCACTCATTTTGGTTCTCTCTCCTGGCGATTAAGTTGATTCTCAAAATGGTTCT > W3110S.gb/1999870‑1999997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |