| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,077,980 | C→T | 100% | Y355Y (TAC→TAT) | yeeR → | predicted membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,077,980 | 0 | C | T | 94.7% | 107.2 / ‑1.1 | 38 | Y355Y (TAC→TAT) | yeeR | predicted membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (15/21); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGTGCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCT > W3110S.gb/2077912‑2078037 | tgtgCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTg > 1:3061255/1‑71 (MQ=255) cctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTg > 1:3328939/1‑64 (MQ=255) cctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTg > 1:3191421/1‑64 (MQ=255) cctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTg > 1:1771992/1‑64 (MQ=255) ctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCatctc > 1:2834907/1‑71 (MQ=255) aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCtt > 1:1326102/1‑63 (MQ=255) aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCtt > 1:3227981/1‑63 (MQ=255) aCAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCtt > 1:3223844/1‑63 (MQ=255) tACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGc < 1:3159182/47‑1 (MQ=255) tACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGc < 1:2550760/47‑1 (MQ=255) tACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGc < 1:239958/47‑1 (MQ=255) aCATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCtt > 1:1922452/1‑55 (MQ=255) aCATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCtt > 1:1329493/1‑55 (MQ=255) tGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgt < 1:2081569/71‑1 (MQ=255) tGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgt < 1:2677172/71‑1 (MQ=255) tGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgt > 1:376165/1‑71 (MQ=255) tGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgt > 1:2008197/1‑71 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3547032/47‑1 (MQ=255) tCATAAGCCGGATCCTGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAttt < 1:3519263/71‑1 (MQ=255) aTAAGCCGGATCCGGAATGGTATTGCTCCTCTCttt < 1:3068282/36‑1 (MQ=255) tAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATg < 1:390672/71‑1 (MQ=255) tAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATg < 1:3689809/71‑1 (MQ=255) tAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATg < 1:3016459/71‑1 (MQ=255) ggATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGcc < 1:397849/71‑1 (MQ=255) ggATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGcc < 1:1517898/71‑1 (MQ=255) ggATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGcc > 1:740377/1‑71 (MQ=255) gATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATg < 1:1833830/64‑1 (MQ=255) aTCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:1069979/40‑1 (MQ=255) aTCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:171176/40‑1 (MQ=255) aTCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacaga < 1:2334628/40‑1 (MQ=255) aaTGGTATTGCTCCTCTCTTTTTCGCGAAACAGAAACACTGTGTCAGGAAATTTATg < 1:2679987/57‑1 (MQ=255) aaTGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCATGAAATTTATg < 1:604585/57‑1 (MQ=255) tGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATg < 1:3340996/55‑1 (MQ=255) tGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATg < 1:2141932/55‑1 (MQ=255) gTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGaag > 1:3309367/1‑55 (MQ=255) gTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:1651259/1‑61 (MQ=255) gTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:2516105/1‑61 (MQ=255) gTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTAAGAACGCCCt > 1:178320/1‑61 (MQ=255) | TGTGCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCT > W3110S.gb/2077912‑2078037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |