| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,129,117 | C→T | 45.2% | A71T (GCC→ACC) | fcl ← | bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase and GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,129,117 | 0 | C | T | 45.2% | 7.1 / 38.3 | 31 | A71T (GCC→ACC) | fcl | bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase and GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase |
| Reads supporting (aligned to +/- strand): ref base C (14/3); new base T (9/5); total (23/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCAT > W3110S.gb/2129050‑2129185 | gcgTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGcaac < 1:2379707/71‑1 (MQ=255)gcgTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGcaac < 1:1561922/71‑1 (MQ=255)gcgTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGcaac < 1:612337/71‑1 (MQ=255)gcgTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGcaac < 1:2219334/71‑1 (MQ=255)gcgTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGcaac < 1:2133653/71‑1 (MQ=255)gcgTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGcaac < 1:3606058/71‑1 (MQ=255)gcgTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCCGATAGGTGTTGTTGGtaac < 1:1113896/71‑1 (MQ=255) gTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGtaacaa < 1:3389578/71‑1 (MQ=255) tGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGtaacaa < 1:2369488/70‑1 (MQ=255) atgTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGGCGGATAGGTGTTGTTGGTAACAATgccgcc > 1:850161/1‑71 (MQ=255) tgTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATgccgcc > 1:2718173/1‑70 (MQ=255) tgTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATgccgcc > 1:2928515/1‑70 (MQ=255) tGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCCCACt > 1:523457/1‑71 (MQ=255) atcatcATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGcaac < 1:513377/50‑1 (MQ=255) atcatcATGTTCTCGTAGATGAAATCCGCCGGATAGGTGTTGTTGGcaac < 1:1014871/50‑1 (MQ=255) atcatGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGtaa > 1:1116498/1‑46 (MQ=255) catGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCCCACTTTcgccgccgc > 1:93287/1‑69 (MQ=255) ttCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACt < 1:3184887/54‑1 (MQ=255) ttCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACt < 1:949304/54‑1 (MQ=255) cTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGcaacaa > 1:3533701/1‑41 (MQ=255) cTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAg < 1:2542620/70‑1 (MQ=255) cTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAg < 1:1986891/70‑1 (MQ=255) tAGATGAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTg > 1:742156/1‑71 (MQ=255) tAGATGAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTg > 1:1986473/1‑71 (MQ=255) aGATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATgccg > 1:141176/1‑41 (MQ=255) gATGAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGa < 1:1521060/61‑1 (MQ=255) gATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGt > 1:849849/1‑71 (MQ=255) gATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGt > 1:3253956/1‑71 (MQ=255) gATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGt > 1:3344754/1‑71 (MQ=255) gATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCACACTTTCGCCGCCGCCAGATAGACCTGGt > 1:2569303/1‑71 (MQ=255) tGAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGAt > 1:268726/1‑60 (MQ=255) tGAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATa > 1:1986408/1‑61 (MQ=255) gAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCaa > 1:2055433/1‑71 (MQ=255) gAAATCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCaa > 1:3163973/1‑71 (MQ=255) gAAATCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCaa > 1:306758/1‑71 (MQ=255) aaaTCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGa > 1:3717540/1‑57 (MQ=255) aaaTCCGCCGGATAGGTGTTGTTGGTAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCaa > 1:1574068/1‑70 (MQ=255) tCCGCCGGATAGGTGTTGTTGGCAACAATgccgcc > 1:1871653/1‑35 (MQ=255) tCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGa > 1:3345072/1‑54 (MQ=255) tAGGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTgg > 1:2660029/1‑70 (MQ=255) gttgGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAAt > 1:335653/1‑51 (MQ=255) ttgGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAAt < 1:3239781/70‑1 (MQ=255) ttgGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAAt < 1:1430936/70‑1 (MQ=255) gCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCAt < 1:2805923/70‑1 (MQ=255) | GCGTGAATGATGTTGCTCTCAATCATCATGTTCTGGTAGATGAAATCCGCCGGATAGGTGTTGTTGGCAACAATGCCGCCCACTTTCGCCGCCGCCAGATAGACCTGGTCAATACGTTCGCTGGCAAAGAAATCAT > W3110S.gb/2129050‑2129185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |