| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,160,543 | C→T | 100% | F7F (TTC→TTT) | mdtC → | multidrug efflux system, subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,160,543 | 0 | C | T | 92.6% | 72.0 / ‑1.0 | 27 | F7F (TTC→TTT) | mdtC | multidrug efflux system, subunit C |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (10/15); total (10/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCATA > W3110S.gb/2160477‑2160600 | aTTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTAttt < 1:1643462/71‑1 (MQ=255) aaaaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACAGCCCGGt < 1:3071322/71‑1 (MQ=255) aaaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTAt > 1:3379774/1‑58 (MQ=255) aaaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTAt > 1:1305416/1‑58 (MQ=255) aaaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTAt > 1:2713120/1‑58 (MQ=255) aaaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTAt > 1:2110536/1‑58 (MQ=255) cTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGcgacga < 1:2612335/71‑1 (MQ=255) cccGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAtttt < 1:1172432/70‑1 (MQ=255) cccGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAtttt < 1:2761476/70‑1 (MQ=255) tCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCtttattta < 1:348455/45‑1 (MQ=255) tCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAtttt < 1:447471/66‑1 (MQ=255) tCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAtttt < 1:2629742/66‑1 (MQ=255) tCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAtttt < 1:1782355/66‑1 (MQ=255) tCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGAtttt < 1:3118469/66‑1 (MQ=255) cATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCtttattta < 1:1972278/44‑1 (MQ=255) aTGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCg > 1:2192404/1‑71 (MQ=255) aTGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCg > 1:2755659/1‑71 (MQ=255) gaggagGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGc < 1:2763073/71‑1 (MQ=255) gaggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAt < 1:3675304/71‑1 (MQ=255) aggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTg > 1:3647881/1‑59 (MQ=255) aggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCa > 1:1842217/1‑69 (MQ=255) aggCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCAt < 1:1741249/70‑1 (MQ=255) tAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTa < 1:2785536/67‑1 (MQ=255) tAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTa < 1:663608/67‑1 (MQ=255) aGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCtgt > 1:1000443/1‑71 (MQ=255) ttttttGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGcc < 1:669708/54‑1 (MQ=255) ttttttGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCAt < 1:1557696/71‑1 (MQ=255) ttttttGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCAt < 1:2500851/71‑1 (MQ=255) tttttGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCATa > 1:643304/1‑71 (MQ=255) | ATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCATA > W3110S.gb/2160477‑2160600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |