| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,193,388 | T→C | 7.1% | Q286R (CAG→CGG) | yehB ← | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,193,388 | 0 | T | C | 7.1% | 156.5 / 3.9 | 56 | Q286R (CAG→CGG) | yehB | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (30/22); new base C (2/2); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
ATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGT > W3110S.gb/2193318‑2193456 | aTCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCt > 1:250188/1‑71 (MQ=255)aTCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCt > 1:1462960/1‑71 (MQ=255) cAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGa > 1:2968673/1‑71 (MQ=255) aaGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGa > 1:3639821/1‑70 (MQ=255) aaGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGa > 1:1700116/1‑70 (MQ=255) aaGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGa > 1:1471029/1‑70 (MQ=255) aaGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCCGa > 1:662187/1‑70 (MQ=255) aGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATa < 1:1985795/71‑1 (MQ=255) cTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATaaa > 1:1509016/1‑69 (MQ=255) cTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAAc > 1:973871/1‑70 (MQ=255) cTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAAc > 1:3078106/1‑70 (MQ=255) cTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAAc > 1:1362803/1‑70 (MQ=255) caccacCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACaa < 1:49154/69‑1 (MQ=255) caccacCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACaa < 1:3605566/69‑1 (MQ=255) caccacCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACaa < 1:208507/69‑1 (MQ=255) accGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACaaaa > 1:80223/1‑67 (MQ=255) accGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACaaaa > 1:712033/1‑67 (MQ=255) accGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACaaaa > 1:1344661/1‑67 (MQ=255) aaCTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGtt > 1:2239224/1‑70 (MQ=255) cAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCCGATAAACCACAAAACCATTCTGTTCAAt < 1:3655299/69‑1 (MQ=255) cAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCCGATAAACCACAAAACCATTCTGTTCAAt < 1:1449754/69‑1 (MQ=255) aaTCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGtt < 1:86042/71‑1 (MQ=255) tAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAg < 1:3265193/70‑1 (MQ=255) tCGGGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCAt < 1:2283839/48‑1 (MQ=255) cgcgAACGGGCCAGGAGGAACCTCTTTCCGATAAACCACAAAACCATTCTGTTCAATAg > 1:2565894/1‑59 (MQ=255) gcgAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTc > 1:1250452/1‑48 (MQ=255) gcgAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTc > 1:1978167/1‑48 (MQ=255) gcgAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTc > 1:1794337/1‑48 (MQ=255) gcgAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGt < 1:542706/70‑1 (MQ=255) gcgAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGt < 1:3252983/70‑1 (MQ=255) gcgAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGt < 1:2298349/70‑1 (MQ=255) aaCGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGtt < 1:1569943/57‑1 (MQ=255) ccAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGc < 1:88254/71‑1 (MQ=255) ggaggaACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAAt > 1:2527186/1‑71 (MQ=255) taggaACCTCTTTCTGATAAACCACAAAACCATTCTg < 1:3414696/36‑1 (MQ=255) gaggaACCTCTTTCTGATAAACCACAAAACCATTCTg < 1:2406041/37‑1 (MQ=255) gaggaACCTCTTTCTGATAAACCACAAAACCATTCTg < 1:2273873/37‑1 (MQ=255) gaggaACCTCTTTCTGATAAACCACAAAACCATTCTg < 1:2260027/37‑1 (MQ=255) gaggaACCTCTTTCTGATAAACCACAAAACCATTCTg < 1:1193604/37‑1 (MQ=255) gaggaACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATc < 1:884390/71‑1 (MQ=255) gaACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAgcgc < 1:638797/52‑1 (MQ=255) gaACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAgcgc < 1:209292/52‑1 (MQ=255) gaACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAgcgc < 1:1066929/52‑1 (MQ=255) aCCTCTTTCTGATAAACCACAAAACCATTGTGTTCAATAGTTACCAGCGCGt > 1:2592499/1‑52 (MQ=255) aCCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGt > 1:3401018/1‑52 (MQ=255) ctctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATcc < 1:1480473/65‑1 (MQ=255) tctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTAcc > 1:2648110/1‑42 (MQ=255) tctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTAcc > 1:1366738/1‑42 (MQ=255) tctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCAc > 1:2709536/1‑71 (MQ=255) tctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCAc > 1:1339010/1‑71 (MQ=255) tctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCAc > 1:2966023/1‑71 (MQ=255) tctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAG‑AATCCCCTGCAcc > 1:1518479/1‑71 (MQ=255) ctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCAcc > 1:3409071/1‑71 (MQ=255) ctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCAcc > 1:3010513/1‑71 (MQ=255) ctTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCAcc > 1:1489883/1‑71 (MQ=255) tCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGt > 1:3674037/1‑71 (MQ=255) tCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGt > 1:1178148/1‑71 (MQ=255) tCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGt > 1:1846446/1‑71 (MQ=255) | ATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGT > W3110S.gb/2193318‑2193456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |