| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,891,664 | Δ1 bp | 100% | intergenic (‑82/+409) | gudP ← / ← ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,891,664 | 0 | T | . | 79.2% | 55.9 / 16.8 | 24 | intergenic (‑82/+409) | gudP/ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base . (19/0); total (21/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CTGGACTGCGACATCGCCAGGAAATCAGAGGTGACGTAGGGTGTTTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTATATCTGGCAATAGCAGTATA > minE/1891621‑1891730 | ctGGACTGCGACATCGCCAGGAAATCAGAGGTGACGTAGGGTGTTTTTTGCCGTTTTTATAGGTCGTTc > 1:3083601/1‑69 (MQ=255) gCGACATCGCCAGGAAATCAGAGGTGACGTAGGGTGTTTTTTGCCGTTTTTATAGGTCGTTCGCCGAAt > 1:2057321/1‑69 (MQ=255) ggAAATCAGAGGTGACGTAGGGTGTTTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGtt < 1:1693847/69‑1 (MQ=255) ggAAATCAGAGGTGACGTAGGGTGTTTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGtt < 1:2220755/69‑1 (MQ=255) aaTCAGAGGTGACGTAGGGTGTTTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTtat < 1:450582/69‑1 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCgt > 1:1664836/1‑61 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTtata > 1:1633722/1‑68 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:2767454/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:976882/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:771952/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:735436/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:700531/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:525547/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:3120676/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:3084036/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:3012862/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:1165277/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:2679102/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:2393358/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:2098551/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:1664933/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:1583148/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTatat > 1:1185871/1‑69 (MQ=255) aTCAGAGGTGACGTAGGGTG‑TTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTCTTTatat > 1:763219/1‑69 (MQ=255) tgtTTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTATATCTGGCAATAGCAGtat > 1:2866848/1‑68 (MQ=255) gtTTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTATATCTGGCAATAGCAGtata > 1:1457259/1‑68 (MQ=255) | CTGGACTGCGACATCGCCAGGAAATCAGAGGTGACGTAGGGTGTTTTTTGCCGTTTTTATAGGTCGTTCGCCGAATACGGCGCGTGTTTATATCTGGCAATAGCAGTATA > minE/1891621‑1891730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |