Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,892,019:1	+GC	100%	intergenic (‑437/+54)	gudP ← / ← ygdL	predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,892,019	1	.	G	100.0%	76.9 / NA	32	intergenic (‑437/+54)	gudP/ygdL	predicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (30/2);  total (30/2)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*	minE	1,892,019	2	.	C	100.0%	77.2 / NA	32	intergenic (‑437/+54)	gudP/ygdL	predicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (30/2);  total (30/2)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTG  >  minE/1891977‑1892071                                            ||                                                     tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCTGGTCGGATaa                              >  1:2286802/1‑69 (MQ=255) tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                              >  1:1325221/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                         >  1:3199404/1‑57 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                               >  1:1695118/1‑67 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:2500991/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:993833/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:3433178/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:3100838/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:2944151/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:1134343/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:2296134/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:1948472/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:1221545/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:1345327/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:1559375/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:1735222/1‑69 (MQ=255)  cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                             >  1:178171/1‑69 (MQ=255)                       tACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                         >  1:2624284/1‑36 (MQ=25)                       tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGt                                     >  1:1560920/1‑40 (MQ=255)                       tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                 >  1:2478175/1‑44 (MQ=255)                       tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGCTAacacc                          >  1:2397569/1‑50 (MQ=255)                       tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGATGTTCGCGTCTCATCCGAc         >  1:2387601/1‑68 (MQ=255)                       tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcg                      >  1:559370/1‑55 (MQ=255)                       tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTc                       >  1:545734/1‑54 (MQ=255)                       tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                   >  1:258228/1‑58 (MQ=255)                       tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGAc         >  1:877146/1‑68 (MQ=255)                          cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTg      <  1:376897/68‑1 (MQ=255)                          cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTg      <  1:896446/68‑1 (MQ=255)                              cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc                           >  1:710809/1‑43 (MQ=37)                              cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgcg                    >  1:3316611/1‑50 (MQ=255)                              cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTc                       >  1:3117455/1‑47 (MQ=37)                              cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTg  >  1:1963500/1‑68 (MQ=38)                                            ||                                                     TCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTG  >  minE/1891977‑1892071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑